Evidence map›Paper›PMID 34669971›Full record

ArticleClinical and experimental dermatology2022

Interleukin-17E, inducible nitric oxide synthase and arginase1 as new biomarkers in the identification of neutrophilic dermatoses.

R Stalder, N Brembilla, C Conrad, N Yawalkar, A Navarini, W H Boehncke, G Kaya

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical and experimental dermatology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it, 10 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Article
  5. Review
  6. Review
  7. Review
  8. New Practical Aspects of Sweet Syndrome.American journal of clinical dermatology · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

R StalderDepartment of Pathology and Immunology, University of Geneva, Geneva, Switzerland.ORCID https://orcid.org/0000-0001-8513-1250
N BrembillaDepartment of Pathology and Immunology, University of Geneva, Geneva, Switzerland.ORCID https://orcid.org/0000-0003-1591-1790
C ConradDepartment of Dermatology, University Hospital CHUV, Lausanne, Switzerland.
N YawalkarDepartment of Dermatology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
A NavariniDepartment of Biomedicine, University Hospital Basel, Basel, Switzerland.
W H BoehnckeDepartment of Pathology and Immunology, University of Geneva, Geneva, Switzerland.
G KayaDepartment of Pathology and Immunology, University of Geneva, Geneva, Switzerland.
University of Geneva · CHUniversity Hospital of Basel · CHUniversity Hospital of Lausanne · CHUniversity of Bern · CH

Funding

Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung 310030-175470/1
6 · The paper itself

Abstract

backgroundNeutrophilic dermatoses (ND) are a heterogeneous group of diseases, but can often have a relatively similar histological appearance.

aimTo identify a combination of biomarkers allowing a better differentiation of ND types.

methodsBiopsies were obtained from normal human skin (NS; n = 4), chronic plaque-type psoriasis (PsO; n = 7), paradoxical psoriasis (PP; n = 8), generalized pustular psoriasis (GPP; n = 9), subcorneal pustular dermatosis of Sneddon-Wilkinson (SPD; n = 3), acute generalized exanthematous pustulosis (AGEP; n = 3), hidradenitis suppurativa (HS; n = 7), Sweet syndrome (SS; n = 8) and pyoderma gangrenosum (PG; n = 8). Samples were analysed by immunofluorescence using three biomarkers, interleukin (IL)-17E, inducible nitric oxide synthase (iNOS) and arginase1, each one in combination with two cell markers, myeloperoxidase (MPO) and CD68, which allow the identification of neutrophils and macrophages, respectively.

resultsWe found that SS is characterized by high expression of IL-17E and iNOS in the epidermis, while PG exhibits low expression. The density of the neutrophil infiltrate helps to differentiate PP (high-density infiltrate) from PsO (low-density infiltrate). High expression of arginase1 in the granular layer of the epidermis is a hallmark of SPD. Finally, mature neutrophils and proinflammatory macrophages are readily detectable in PP, SPD and PG, whereas immature neutrophils and anti-inflammatory macrophages are more frequent in GPP, AGEP, HS and SS.

conclusionsThe analysis of ND by immunofluorescence using IL-17E, iNOS and arginase1 in combination with MPO and CD68 allows for characterization of differential expression patterns in the epidermis as well as the determination of the polarization status of the dermal neutrophils and macrophages. The appropriate markers may help in the differentiation of ND in clinical practice.

Indexed as

DermatitisPsoriasisArginaseBiomarkersHumansInterleukin-17Nitric Oxide Synthase Type IIARG1 protein, humanArginaseBiomarkersIL25 protein, humanInterleukin-17Nitric Oxide Synthase Type IINOS2 protein, human

Identifiers

PMID34669971
PMCPMC9300036
OpenAlexW3205528657

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.