Evidence map›Paper›PMID 34669399›Full record

ArticleJournal of proteome research2021

Adsorptive Microtiter Plates As Solid Supports in Affinity Purification Workflows.

Frank Klont, Marcel Kwiatkowski, Alen Faiz, Thea van den Bosch, Simon D Pouwels, Frank J Dekker, Nick H T Ten Hacken, Péter Horvatovich, Rainer Bischoff

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of proteome research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Frank KlontDepartment of Analytical Biochemistry, Groningen Research Institute of Pharmacy, University of Groningen, 9700 AB Groningen, The Netherlands.ORCID 0000-0003-3503-1694
Marcel KwiatkowskiDepartment of Analytical Biochemistry, Groningen Research Institute of Pharmacy, University of Groningen, 9700 AB Groningen, The Netherlands.ORCID 0000-0002-5804-6031
Alen FaizDepartment of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, 9700 AB Groningen, The Netherlands.
Thea van den BoschDepartment of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, 9700 AB Groningen, The Netherlands.
Simon D PouwelsDepartment of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, 9700 AB Groningen, The Netherlands.
Frank J DekkerDepartment of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, 9700 AB Groningen, The Netherlands.ORCID 0000-0001-7217-9300
Nick H T Ten HackenDepartment of Pulmonary Diseases, University Medical Center Groningen, University of Groningen, 9700 AB Groningen, The Netherlands.
Péter HorvatovichDepartment of Analytical Biochemistry, Groningen Research Institute of Pharmacy, University of Groningen, 9700 AB Groningen, The Netherlands.ORCID 0000-0003-2218-1140
Rainer BischoffDepartment of Analytical Biochemistry, Groningen Research Institute of Pharmacy, University of Groningen, 9700 AB Groningen, The Netherlands.ORCID 0000-0001-9849-0121
University of Groningen · NLUniversity Medical Center Groningen · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Affinity ligands such as antibodies are widely used in (bio)medical research for purifying proteins from complex biological samples. These ligands are generally immobilized onto solid supports which facilitate the separation of a captured protein from the sample matrix. Adsorptive microtiter plates are commonly used as solid supports prior to immunochemical detection (e.g., immunoassays) but hardly ever prior to liquid chromatography-mass spectrometry (LC-MS-)-based detection. Here, we describe the use of adsorptive microtiter plates for protein enrichment prior to LC-MS detection, and we discuss opportunities and challenges of corresponding workflows, based on examples of targeted (i.e., soluble receptor for advanced glycation end-products (sRAGE) in human serum) and discovery-based workflows (i.e., transcription factor p65 (NF-κB) in lysed murine RAW 264.7 macrophages and peptidyl-prolyl cis-trans isomerase FKBP5 (FKBP5) in lysed human A549 alveolar basal epithelial cells). Thereby, we aim to highlight the potential usefulness of adsorptive microtiter plates in affinity purification workflows prior to LC-MS detection, which could increase their usage in mass spectrometry-based protein research.

Indexed as

WorkflowAnimalsChromatography, AffinityChromatography, LiquidHumansMass SpectrometryMiceReceptor for Advanced Glycation End ProductsReceptor for Advanced Glycation End Products

Identifiers

PMID34669399
PMCPMC8576839
OpenAlexW3205716309

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.