Evidence map›Paper›PMID 34669264›Full record

ArticleJournal of diabetes investigation2022

Immunohistochemical detection of enteroviruses in pancreatic tissues of patients with type 1 diabetes using a polyclonal antibody against 2A protease of Coxsackievirus.

Erika Jimbo, Tetsuro Kobayashi, Akira Takeshita, Keiichiro Mine, Seiho Nagafuchi, Tomoyasu Fukui, Soroku Yagihashi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of diabetes investigation, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Erika JimboDivision of Immunology and Molecular Medicine, Okinaka Memorial Institute for Medical Research, Tokyo, Japan.
Tetsuro KobayashiDivision of Immunology and Molecular Medicine, Okinaka Memorial Institute for Medical Research, Tokyo, Japan.ORCID https://orcid.org/0000-0002-4364-2527
Akira TakeshitaDepartment of Endocrinology and Metabolism, Toranomon Hospital, Tokyo, Japan.
Keiichiro MineDivision of Metabolism and Endocrinology, Department of Internal Medicine, Faculty of Medicine, Saga University, Saga, Japan.ORCID https://orcid.org/0000-0001-9759-4148
Seiho NagafuchiDivision of Metabolism and Endocrinology, Department of Internal Medicine, Faculty of Medicine, Saga University, Saga, Japan.
Tomoyasu FukuiDivision of Diabetes, Metabolism and Endocrinology, Department of Medicine, Showa University School of Medicine, Tokyo, Japan.
Soroku YagihashiDepartment of Exploratory Medicine on Nature, Life, and Man, Toho University School of Medicine, Chiba, Japan.ORCID https://orcid.org/0000-0003-4255-774X
Saga University · JPToranomon Hospital · JPShowa University · JPToho University · JP

Funding

Japan Society for Promotion of Science KAKENHI 15K09406Japan Society for Promotion of Science KAKENHI 21K08541
6 · The paper itself

Abstract

AIMS/

introductionThe need for antiserum for immunohistochemical (IHC) detection of enterovirus (EV) in formaldehyde-fixed and paraffin-embedded samples is increasing. The gold standard monoclonal antibody (clone 5D8/1) against EV-envelope protein (VP1) was proven to cross-react with other proteins. Another candidate marker of EV proteins is 2A protease (2A MATERIALS AND

methodsWe raised polyclonal antiserum by immunizing a rabbit with an 18-mer peptide of Coxsackievirus B1 (CVB1)-2A

resultsEnzyme-linked immunosorbent assay study showed a high titer of antibody for 18-mer peptide of CVB1-2A

conclusionsOur study provides a new polyclonal antiserum against CVB1-2A

Indexed as

Diabetes Mellitus, Type 1EnterovirusAnimalsChlorocebus aethiopsHumansPancreasPeptide HydrolasesRabbitsVero CellsPeptide Hydrolases2A proteaseEnterovirusPolyclonal antiserum

Identifiers

PMID34669264
PMCPMC8902398
OpenAlexW3206856485

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.