ArticlePlant biotechnology journal2022
CRISPR-BETS: a base-editing design tool for generating stop codons.
Article in Plant biotechnology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 40 citations in OpenAlex.
- Enhancing CRISPR-Cas12a base editing in plants with LbCas12a variants and introns.Journal of integrative plant biology · 2026Article
- Populus PtrbHLH011 Is a Transcriptional Co-Regulator Involved in the Activation of Cell Wall Biosynthesis by Iron Deprivation.Plant biotechnology journal · 2025Article
- Transgene-free genome editing in citrus and poplar trees using positive and negative selection markers.Plant cell reports · 2025Article
- CRISPR-GATE: a one-stop repository and guide to computational resources for genome editing experimentation.Briefings in bioinformatics · 2025Review
- Research Progress on the Regulatory Mechanism of theCurrent issues in molecular biology · 2025Review
- QBEmax redefines the precise base editing in crop plants.Functional & integrative genomics · 2025Article
- Current trends in gene therapy to treat inherited disorders of the brain.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Review
- Gene Editing inJournal of fungi (Basel, Switzerland) · 2025Review
- BES-Designer: A Web Tool to Design Guide RNAs for Base Editing to Simplify Library.Interdisciplinary sciences, computational life sciences · 2025Article
- PAM-relaxed and temperature-tolerant CRISPR-Mb3Cas12a single transcript unit systems for efficient singular and multiplexed genome editing in rice, maize, and tomato.Plant biotechnology journal · 2025Article
- Versatile plant genome engineering using anti-CRISPR-Cas12a systems.Science China. Life sciences · 2024Article
- Review
- High performance TadA-8e derived cytosine and dual base editors with undetectable off-target effects in plants.Nature communications · 2024Article
- Boosting genome editing in plants with single transcript unit surrogate reporter systems.Plant communications · 2024Article
- Review
- Recent Advances in Studying the Regulation of Fruit Ripening in Tomato Using Genetic Engineering Approaches.International journal of molecular sciences · 2024Review
- Efficient plant genome engineering using a probiotic sourced CRISPR-Cas9 system.Nature communications · 2023Article
- CRISPR-Combo-mediated orthogonal genome editing and transcriptional activation for plant breeding.Nature protocols · 2023Review
- An efficient CRISPR-Cas12a promoter editing system for crop improvement.Nature plants · 2023Article
- Designing Guide-RNA for Generating Premature Stop Codons for Gene Knockout Using CRISPR-BETS.Methods in molecular biology (Clifton, N.J.) · 2023Article
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytosine base editors (CBEs) can install a predefined stop codon at the target site, representing a more predictable and neater method for creating genetic knockouts without altering the genome size. Due to the enhanced predictability of the editing outcomes, it is also more efficient to obtain homozygous mutants in the first generation. With the recent advancement of CBEs on improved editing activity, purify and specificity in plants and animals, base editing has become a more appealing technology for generating knockouts. However, there is a lack of design tools that can aid the adoption of CBEs for achieving such a purpose, especially in plants. Here, we developed a user-friendly design tool named CRISPR-BETS (base editing to stop), which helps with guide RNA (gRNA) design for introducing stop codons in the protein-coding genes of interest. We demonstrated in rice and tomato that CRISPR-BETS is easy-to-use, and its generated gRNAs are highly specific and efficient for generating stop codons and obtaining homozygous knockout lines. While we tailored the tool for the plant research community, CRISPR-BETS can also serve non-plant species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.