ArticleMicrobiology spectrum2021
Comparative Analysis of the Cultured and Total Bacterial Community in the Wheat Rhizosphere Microbiome Using Culture-Dependent and Culture-Independent Approaches.
Article in Microbiology spectrum, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Challenges and Future Directives of Synthetic Biology in Engineering Plant-Microbe Partnerships for Sustainable Agriculture.Biotechnology and bioengineering · 2026Review
- Phyllosphere microbiomes in grassland plants harbor a vast reservoir of novel antimicrobial peptides and biosynthetic diversity.Journal of advanced research · 2026Article
- Beneficial microbes isolated from the strawberry rhizosphere: development of growth-promoting and anthracnose-suppressive SynComs.World journal of microbiology & biotechnology · 2026Article
- Neighborhood deadwood and yard rewilding modulate commensal microbiomes and inflammatory signals among urbanites.Microbiome · 2026Article
- Sulfur Oxidation by New and Non-Canonical Bacteria in a Subsurface Flow Constructed Wetland Treating Domestic Wastewater.Microorganisms · 2026Article
- Indigenous Olive Orchard Bacteria as Biocontrol Agents: An Integrated Culture-Dependent and Soil Microbiome Approach.Microorganisms · 2026Article
- Metagenomic Tools as Predictive Approaches to Decipher Soil and Rhizosphere Microbial Communities and Their Functions for Sustainable Agriculture.International journal of genomics · 2026Review
- Endophytic bacterial communities associated with halophytic plants in kebili and Gabes regions of Southern Tunisia.BMC microbiology · 2025Article
- Defining the cultured and uncultured bacterial fractions in Cannabis seeds.Environmental microbiome · 2025Article
- Host genotype-driven shifts in theFrontiers in microbiology · 2025Article
- Short-term dynamics of fecal microbiome and antibiotic resistance in juvenile rainbow trout (Oncorhynchus mykiss) following antibiotic treatment and withdrawal.Animal microbiome · 2024Article
- Comparative Genomics and Biosynthetic Cluster Analysis of Antifungal Secondary Metabolites of Three Strains ofMicroorganisms · 2024Article
- Complete genome ofMicrobiology resource announcements · 2024Article
- Metagenomic evaluation of peanut rhizosphere microbiome from the farms of Saurashtra regions of Gujarat, India.Scientific reports · 2024Article
- Comparative analysis of the microbiomes of strawberry wild speciesFrontiers in microbiology · 2024Article
- Microbial Assemblages Associated with the Soil-Root Continuum of an Endangered Plant,Microbiology spectrum · 2023Article
- Culture-Dependent and Metabarcoding Characterization of the Sugar Beet (Microorganisms · 2023Article
- Bacterial Communities and Diversity of Western Ghats Soil: A Study of a Biodiversity Hotspot.Current microbiology · 2023Article
- Microbiome Signature of Endophytes in Wheat Seed Response to Wheat Dwarf Bunt Caused by Tilletia controversa Kühn.Microbiology spectrum · 2023Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhizosphere and root-associated bacteria are key components of crop production and sustainable agriculture. However, utilization of these beneficial bacteria is often limited by conventional culture techniques because a majority of soil microorganisms cannot be cultured using standard laboratory media. Therefore, the purpose of this study was to improve culturability and investigate the diversity of the bacterial communities from the wheat rhizosphere microbiome collected from three locations in Egypt with contrasting soil characteristics by using metagenomic analysis and improved culture-based methods. The improved strategies of the culture-dependent approach included replacing the agar in the medium with gellan gums and modifying its preparation by autoclaving the phosphate and gelling agents separately. Compared to the total operational taxonomic units (OTUs) observed from the metagenomic data sets derived from the three analyzed soils, 1.86 to 2.52% of the bacteria were recovered using the modified cultivation strategies, whereas less than 1% were obtained employing the standard cultivation protocols. Twenty-one percent of the cultivable isolates exhibited multiple plant growth-promoting (PGP) properties, including P solubilization activity and siderophore production. From the metagenomic analysis, the most abundant phyla were
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