ArticlePloS one2021
Improvement of 2-phenylethanol production in Saccharomyces cerevisiae by evolutionary and rational metabolic engineering.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Triparental synthetic yeast hybrids as a platform for higher 2-phenylethanol yields and stress resistance.World journal of microbiology & biotechnology · 2026Article
- Fermentation Process Optimization for High 2-Phenylethanol Aroma Whisky.International journal of molecular sciences · 2026Article
- Systematic process optimization for enhanced biosynthesis of 2-phenylethanol using Candida sorboxylosa.Bioprocess and biosystems engineering · 2026Article
- High-titer 2-phenylethanol production in Escherichia coli via decarboxylation-step optimization and a vegetable-oil overlay for biocompatible in situ recovery.Journal of biological engineering · 2026Article
- EngineeringFrontiers in microbiology · 2026Review
- Endogenous pathway dysregulation in Kluyveromyces marxianus under nitrogen limitation drives 2-phenylethyl acetate and 2-phenylethanol overproduction.Scientific reports · 2025Article
- Screening of High-Yield 2-Phenylethanol Producing Strain from Wild-TypeFoods (Basel, Switzerland) · 2025Article
- Enhanced multistress tolerance ofMicrobiology spectrum · 2025Article
- Yeast-secreted compounds with antifungal activity-screening, genetic parts, biosynthetic pathways, and regulation.FEMS yeast research · 2025Review
- Microbial engineering for monocyclic aromatic compounds production.FEMS microbiology reviews · 2025Review
- Engineering the L-tryptophan metabolism for efficient de novo biosynthesis of tryptophol in Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2024Article
- Identification of transporters involved in aromatic compounds tolerance through screening of transporter deletion libraries.Microbial biotechnology · 2024Article
- Isolation and Identification of Non-Journal of fungi (Basel, Switzerland) · 2023Article
- Article
- Genomic, transcriptomic, and metabolic characterization of 2-Phenylethanol-resistantFrontiers in microbiology · 2023Article
- Evolutionary and reverse engineering in Saccharomyces cerevisiae reveals a Pdr1p mutation-dependent mechanism for 2-phenylethanol tolerance.Microbial cell factories · 2022Article
- Combining metabolite doping and metabolic engineering to improve 2-phenylethanol production by engineered cyanobacteria.Frontiers in bioengineering and biotechnology · 2022Article
- Bioproduction of 2-Phenylethanol through Yeast Fermentation on Synthetic Media and on Agro-Industrial Waste and By-Products: A Review.Foods (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
2-Phenylethanol (2-PE) is a valuable aromatic compound with favorable flavors and good properties, resulting in its widespread application in the cosmetic, food and medical industries. In this study, a mutant strain, AD032, was first obtained by adaptive evolution under 2-PE stress. Then, a fusion protein from the Ehrlich pathway, composed of tyrB from Escherichia coli, kdcA from Lactococcus lactis and ADH2 from Saccharomyces cerevisiae, was constructed and expressed. As a result, 3.14 g/L 2-PE was achieved using L-phenylalanine as a precursor. To further increase 2-PE production, L-glutamate oxidase from Streptomyces overexpression was applied for the first time in our research to improve the supply of α-ketoglutarate in the transamination of 2-PE synthesis. Furthermore, we found that the disruption of the pyruvate decarboxylase encoding gene PDC5 caused an increase in 2-PE production, which has not yet been reported. Finally, assembly of the efficient metabolic modules and process optimization resulted in the strain RM27, which reached 4.02 g/L 2-PE production from 6.7 g/L L-phenylalanine without in situ product recovery. The strain RM27 produced 2-PE (0.8 mol/mol) with L-phenylalanine as a precursor, which was considerably high, and displayed manufacturing potential regarding food safety and process simplification aspects. This study suggests that innovative strategies regarding metabolic modularization provide improved prospects for 2-PE production in food exploitation.
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