ArticleThe American journal of tropical medicine and hygiene2021
Development and Efficacy of Droplet Digital PCR for Detection of Strongyloides stercoralis in Stool.
Article in The American journal of tropical medicine and hygiene, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Systematic Review of Strongyloides stercoralis Infection Diagnosis in Southeast Asia: Insights from Parasitological, Molecular, and Serological Approaches.The American journal of tropical medicine and hygiene · 2024Pooled it
- Current and emerging molecular diagnostic approaches in the detection of human parasites.Parasitology research · 2026Review
- An Agar-Free, Glass Bead-Based Method for the Culture ofDiagnostics (Basel, Switzerland) · 2026Article
- Strongyloides Infection Presenting asTropical medicine and infectious disease · 2025Article
- Partially purifiedMicrobiology spectrum · 2025Article
- Concurrent strongyloidiasis and allergic bronchopulmonary aspergillosis complicating diagnosis: case report.Frontiers in medicine · 2025Article
- Evaluation of Molecular Assays for Diagnosis of Amoebic Liver Abscess in India with Bayesian Latent Class Analysis.The American journal of tropical medicine and hygiene · 2024Article
- Application of Nested-qPCR-High Resolution Melting (HRM) Technology on Strongyloides stercoralis Isolates from Iran.Acta parasitologica · 2024Article
- Examination of Diagnostic Performance of New IgG4 Rapid Test Compared with IgG- and IgG4-ELISAs to Investigate Epidemiology of Strongyloidiasis in Northeast Thailand.The American journal of tropical medicine and hygiene · 2024Article
- Strongyloidiasis.Nature reviews. Disease primers · 2024Review
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- Article
- Diagnostic performance of Strongyloides-specific IgG4 detection in urine for diagnosis of human strongyloidiasis.Parasites & vectors · 2023Article
- From past to present: opportunities and trends in the molecular detection and diagnosis of Strongyloides stercoralis.Parasites & vectors · 2023Review
- Chronic Strongyloides stercoralis infection increases presence of the Ruminococcus torques group in the gut and alters the microbial proteome.Scientific reports · 2023Article
- Improved diagnostic sensitivity of human strongyloidiasis using point-of-care mixed recombinant antigen-based immunochromatography.Parasite (Paris, France) · 2023Article
- Development of a droplet digital polymerase chain reaction tool for the detection of Toxoplasma gondii in meat samples.Parasitology research · 2022Article
- Preliminary study of a prototype fatty acid and retinal binding peptide epitope forTropical parasitologyArticle
- Genetic Diversity ofIranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human strongyloidiasis is one of the neglected tropical diseases caused by infection with soil-transmitted helminth Strongyloides stercoralis. Conventional stool examination, a method commonly used for diagnosis of S. stercoralis, has low sensitivity, especially in the case of light infections. Herein, we developed the droplet digital polymerase chain reaction (ddPCR) assay to detect S. stercoralis larvae in stool and compared its performance with real-time PCR and stool examination techniques (formalin ethyl-acetate concentration technique [FECT] and agar plate culture [APC]). The ddPCR results showed 98% sensitivity and 90% specificity, and real-time PCR showed 82% sensitivity and 76.7% specificity when compared with the microscopic methods. Moreover, ddPCR could detect a single S. stercoralis larva in feces, and cross-reactions with other parasites were not observed. In conclusion, a novel ddPCR method exhibited high sensitivity and specificity for detection of S. stercoralis in stool samples. This technique may help to improve diagnosis, particularly in cases with light infection. In addition, ddPCR technique might be useful for screening patients before starting immunosuppressive drug therapy, and follow-up after treatment of strongyloidiasis.
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