Evidence map›Paper›PMID 34660855›Full record

ArticleRegenerative therapy2021

Long-term culture of rat hepatocytes using human amniotic membrane as a culture substrate.

Kyoichiro Maekawa, Koji Natsuda, Masaaki Hidaka, Masafumi Uematsu, Akihiko Soyama, Takanobu Hara, Mitsuhisa Takatsuki, Kazuhiro Nagai, Kiyonori Miura, Susumu Eguchi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Regenerative therapy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kyoichiro MaekawaDepartment of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Koji NatsudaDepartment of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Masaaki HidakaDepartment of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Masafumi UematsuDepartment of Ophthalmology and Visual Sciences, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Akihiko SoyamaDepartment of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Takanobu HaraDepartment of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Mitsuhisa TakatsukiDepartment of Digestive and General Surgery, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.
Kazuhiro NagaiTransfusion and Cell Therapy Unit, Nagasaki University Hospital, Nagasaki, Japan.
Kiyonori MiuraDepartment of Obstetrics and Gynecology, Nagasaki University Graduate School of BioMedical Sciences, Nagasaki, Japan.
Susumu EguchiDepartment of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Amniotic membrane is attracting attention as a new material for regenerative medicine. We herein report that the culture of primary rat hepatocytes on human amniotic membrane maintained their morphology and their production of albumin for at least two months. Human amniotic membrane was collected during planned cesarean section and kept frozen until usage. Primary rat hepatocytes were plated on human amniotic membrane. Hepatocytes accumulated as colonies on amniotic membrane, and their rat albumin level was maintained for two months. Their three-dimensional structure on extracellular matrix, which is abundant in amniotic membranes might influence the maintenance of the hepatocyte-specific function.

Indexed as

Albumin synthesisAM, amniotic membraneDMSO, dimethyl sulfoxideEGF, epidermal growth factorELISA, enzyme-linked immunosorbent assayFBS, fetal bovine serumHBV, hepatitis-B virusHCV, hepatitis-C virusHGF, hepatocyte growth factorHIV, human immunodeficiency virusHTLV-1, human T-cell leukemia virus type 1Human amniotic membraneLT, liver transplantationPBS, phosphate-buffered salineRat hepatocyte

Identifiers

PMID34660855
PMCPMC8488178

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.