ArticleFrontiers in bioengineering and biotechnology2021
Rapid Separation of Human Hemoglobin on a Large Scale From Non-clarified Bacterial Cell Homogenates Using Molecularly Imprinted Composite Cryogels.
Article in Frontiers in bioengineering and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed, 8 citations in OpenAlex.
- Cryogel-Immobilized Catalase as a Biocatalyst with Enhanced Stability Against Microplastics.Gels (Basel, Switzerland) · 2025Article
- Interaction of haemin with albumin-based macroporous cryogel: Adsorption isotherm and fluorescence quenching studies.Frontiers in bioengineering and biotechnology · 2022Article
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4 authors at 1 institution in 1 country.
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Abstract
The production of a macroporous hydrogel column, known as cryogel, has been scaled up (up to 150 mL) in this work for the purification of human hemoglobin from non-clarified bacterial homogenates. Composite cryogels were synthesized in the presence of adult hemoglobin (HbA) to form a molecularly imprinted polymer (MIP)network where the affinity sites for the targeted molecule were placed directly on an acrylamide cryogel by protein imprinting during the cryogelation. The MIP composite cryogel column was first evaluated in a well-defined protein mixture. It showed high selectivity toward HbA in spite of the presence of serum albumin. Also, when examined in complex non-clarified
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