ArticleFrontiers in cellular and infection microbiology2021
Microbiome Compositions From Infertile Couples Seeking
Article in Frontiers in cellular and infection microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 2 syntheses or guidelines pooled it, 48 citations in OpenAlex.
- Is Lactobacillus spp. beneficial in human semen? Systematic review.JBRA assisted reproduction · 2025Pooled it
- Microbiota and seminal quality: A systematic review.JBRA assisted reproduction · 2023Pooled it
- Unfolding the Semen Microbiota: Implications for Male Infertility.Biomedicines · 2026Review
- Lifestyle factors impact sperm microbiota and are associated with biological and clinical issues in infertile patients undergoing assisted reproductive technologies, a proof-of-concept study.Scientific reports · 2026Article
- Lactobacillus iners at the nexus of microbiota, immunity, and pregnancy.Journal of reproductive immunology · 2026Review
- Decoding the seminal microbial fingerprint and semen quality: insights from the first Greek pilot study.Frontiers in microbiology · 2026Article
- Vaginal microbial community state types fail to predict IVF outcomes, whereasHuman reproduction open · 2026Article
- Retrospective comparative analysis of pre-IVF vaginal and semen microbiological cultures in infertile couples highlights absence of microbial concordance.Frontiers in cellular and infection microbiology · 2026Article
- The overlooked role of the seminovaginal microbiota in infertility: a narrative mini review.Frontiers in medicine · 2026Review
- Exploring Insights Into Vaginal Microbiome Profiles in Relation to Intrauterine Insemination Success Rates.Cureus · 2025Article
- Lifestyle and Environmental Factors Affecting Male Fertility, Individual Predisposition, Prevention, and Intervention.International journal of molecular sciences · 2025Review
- All Properties of Infertility Microbiome in a Review Article.Journal of clinical laboratory analysis · 2025Review
- Semen Microbiome, Male Infertility, and Reproductive Health.International journal of molecular sciences · 2025Review
- Understanding the Associations of Urogenital Microbiomes With Fertility and In Vitro Fertilization.American journal of reproductive immunology (New York, N.Y. : 1989) · 2025Review
- Lower Semen Quality Among Men in the Modern Era-Is There a Role for Diet and the Microbiome?Microorganisms · 2025Review
- State-of-the-art approaches in the investigation of human seminal bacteriome using metagenomic methods.Frontiers in reproductive health · 2025Review
- Vaginal and endometrial microbiome dysbiosis associated with adverse embryo transfer outcomes.Reproductive biology and endocrinology : RB&E · 2024Article
- Contribution of the seminal microbiome to paternal programming.Biology of reproduction · 2024Review
- Unraveling the Intricacies of the Seminal Microbiome and Its Impact on Human Fertility.Biology · 2024Review
- Markers of fertility in reproductive microbiomes of male and female endangered black-footed ferrets (Mustela nigripes).Communications biology · 2024Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Bacterial infections are usually suspected in infertile couples seeking IVF with no clear understanding of the microbial compositions present in the seminal fluids and vaginal niche of the patients. We used next-generation sequencing technology to correlate microbiota compositions with IVF clinical outcomes. Methods: Thirty-six couples were recruited to provide seminal fluids and vaginal swabs. Bacterial DNA was extracted, and V4 region of the 16S rRNA was amplified and sequenced in a pair-end configuration on the Illumina MiSeq platform rendering 2 × 150 bp sequences. Microbial taxonomy to species level was generated using the Greengenes database. Linear discriminant analysis (LDA) effect size (LEfSe) was used to identify biologically and statistically significant differences in relative abundance. Results: Seminal fluid microbiota compositions had lower bacterial concentrations compared with the vagina, but species diversity was significantly higher in seminal fluid samples. Azoospermic subjects had more relative abundance of Conclusions: Semen samples with positive IVF were significantly colonized by
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.