ArticlePostepy dermatologii i alergologii2021
Analysis of the influence of adalimumab to the expression pattern of mRNA and protein of TGF-β1-3 in dermal fibroblast exposed to lipopolysaccharide.
Article in Postepy dermatologii i alergologii, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
introductionPsoriasis is a inflammatory illness, where incorrect expression of cytokines and bacteria lipopolysaccharide are observed. In the therapy of moderate to severe psoriasis anti-TNF drugs, i.e. adalimumab are used which have the influence for secreting another cytokines, such as transforming growth factor-β (TGF-β).
aimTo analyse the expression profile of mRNA MATERIAL AND
methodsNHDFs treated with bacterial lipopolysaccharide at a medium concentration of 1 μg/ml for 8 h, and then added to an adalimumab culture at a concentration of 8 μg/ml and continued exposure of the fibroblasts to it for 2, 8 and 24 h. The molecular analysis included microarray, RTqPCR and ELISA assays.
resultsTreating the skin fibroblast cells with LPS resulted in significant statistical changes in the expression of TGF-β1 (↑) and TGF-β2 (↓) in comparison to the control culture. Likewise, after adding adalimumab to the culture of NHDF treated previously with LPS, significant changes in the expression of TGF-β1 (↑) and TGF-β2 (↓) were noted in comparison to the control culture (
conclusionsBlocking the signalling dependant on TNF-α using adalimumab causes an increase in the expression of TGF-β1 and a simultaneous decrease in the case of TGF-β2.
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