Evidence map›Paper›PMID 34658344›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2021

[An optimized protocol of meniscus cell extraction for single-cell RNA sequencing].

N Chen, H Wu, Z Deng, Z Liao, S Feng, Z Luo, Y Chu, G Qiu, X Li, Y Jin and 5 more

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Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

15 authors.

N ChenDepartment of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
H WuZhujiang Hospital, Southern Medical University, Guangzhou 510080, China.
Z DengDepartment of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Z LiaoDepartment of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
S FengDepartment of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Z LuoDepartment of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Y ChuDepartment of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
G QiuDepartment of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
X LiDepartment of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Y JinDepartment of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
S RongDepartment of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
F WangDepartment of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
L GanDepartment of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
R ChenDepartment of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
L ZhaoDepartment of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo optimize the protocol of meniscus cell extraction to enhance the efficiency of cell suspension preparation and maintain a high cell viability for single-cell RNA sequencing.

methodsWe compared the efficiency of the routine cell extraction methods (short-time digestion and long-time digestion) and the optimized protocol for obtaining meniscus cell suspensions by evaluating the cell number obtained and the cell viability. Single-cell RNA sequencing datasets were analyzed to evaluate the stability of the cell suspension prepared using the optimized protocol. The reliability of the optimized protocol was assessed by comparing the single-cell RNA sequencing dataset obtained by the optimized protocol with published single-cell RNA sequencing datasets of the meniscus.

resultsThe optimized protocol harvested a greater number of cells (over 1×10

conclusionSingle-cell suspension prepared from the meniscus can be stably obtained using the optimized protocol for single-cell RNA sequencing using the 10× Genomics platform.

Indexed as

MeniscusReproducibility of ResultsSequence Analysis, RNAcell extractionmeniscussingle-cell RNA sequencingsingle-cell suspension

Identifiers

PMID34658344
PMCPMC8526306

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