ArticleMolecular biology reports2021
RPS13, a potential universal reference gene for normalisation of gene expression in multiple human normal and cancer tissue samples.
Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 7 citations in OpenAlex.
- The Role of Lymph-Adipose Crosstalk in Alcohol-Induced Perilymphatic Adipose Tissue Dysfunction.International journal of molecular sciences · 2024Article
- Deregulation of Metalloproteinase Expression in Gray Horse Melanoma Ex Vivo and In Vitro.Cells · 2024Article
- Article
- LRP10, PGK1 and RPLP0: Best Reference Genes in Periprostatic Adipose Tissue under Obesity and Prostate Cancer Conditions.International journal of molecular sciences · 2023Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundReference genes are considered stable genes and are used for normalizing the gene expression profile across different cell types; as well as, in normal and diseased samples. However, these gene associates with different biological processes, and hence expression vary in different pathological conditions. Therefore, in the present study, eight different reference genes were used and compared to identify common reference gene usable for an array of different cell types and human cancers. METHODS AND
resultsThe expression stability of the eight reference genes across eleven normal and cancerous tissues was confirmed through real time-qPCR. Ribosomal protein S13 (RPS13) was found to be a common and stable reference gene across intra- and inter-comparison between various normal and tumor tissue types. Further, TCGA data analysis across and between normal and tumor tissue types also showed minimum deviation in expression of RPS13 gene out of eight routinely used reference genes.
conclusionRPS13 is the common stable reference gene in normalization for gene expression based analysis in cancer research.
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