Evidence map›Paper›PMID 34655524›Full record

ArticleDevelopmental cell2021

Adaptable P body physical states differentially regulate bicoid mRNA storage during early Drosophila development.

M Sankaranarayanan, Ryan J Emenecker, Elise L Wilby, Marcus Jahnel, Irmela R E A Trussina, Matt Wayland, Simon Alberti, Alex S Holehouse, Timothy T Weil

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
3.1field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 51 citations in OpenAlex.

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  18. Greatwall-Endos-PP2A/B55Open biology · 2024
    Article
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  20. P-body-like condensates in the germline.Seminars in cell & developmental biology · 2024
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 3 countries.

M SankaranarayananDepartment of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK. Electronic address: msnsankar18@gmail.com.
Ryan J EmeneckerDepartment of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 S. Euclid Ave., St. Louis, MO 63110, USA; Center for Science and Engineering of Living Systems, Washington University in St. Louis, 1 Brookings Drive, St. Louis, MO 63130, USA.
Elise L WilbyDepartment of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK.
Marcus JahnelCenter for Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Tatzberg 47/49, 01307 Dresden, Germany.
Irmela R E A TrussinaCenter for Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Tatzberg 47/49, 01307 Dresden, Germany.
Matt WaylandDepartment of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK.
Simon AlbertiCenter for Molecular and Cellular Bioengineering, Biotechnology Center, Technische Universität Dresden, Tatzberg 47/49, 01307 Dresden, Germany.
Alex S HolehouseDepartment of Biochemistry and Molecular Biophysics, Washington University School of Medicine, 660 S. Euclid Ave., St. Louis, MO 63110, USA; Center for Science and Engineering of Living Systems, Washington University in St. Louis, 1 Brookings Drive, St. Louis, MO 63130, USA.
Timothy T WeilDepartment of Zoology, University of Cambridge, Downing Street, Cambridge CB2 3EJ, UK. Electronic address: tw419@cam.ac.uk.
University of Cambridge · GBTechnische Universität Dresden · DEWashington University in St. Louis · US

Funding

Biotechnology and Biological Sciences Research CouncilWellcome TrustWellcome Trust 097814Wellcome Trust 200734/Z/16/Z
6 · The paper itself

Abstract

Ribonucleoprotein condensates can exhibit diverse physical states in vitro and in vivo. Despite considerable progress, the relevance of condensate physical states for in vivo biological function remains limited. Here, we investigated the physical properties of processing bodies (P bodies) and their impact on mRNA storage in mature Drosophila oocytes. We show that the conserved DEAD-box RNA helicase Me31B forms viscous P body condensates, which adopt an arrested physical state. We demonstrate that structurally distinct proteins and protein-protein interactions, together with RNA, regulate the physical properties of P bodies. Using live imaging and in situ hybridization, we show that the arrested state and integrity of P bodies support the storage of bicoid (bcd) mRNA and that egg activation modulates P body properties, leading to the release of bcd for translation in the early embryo. Together, this work provides an example of how physical states of condensates regulate cellular function in development.

Indexed as

AnimalsBody PatterningDEAD-box RNA HelicasesDrosophilaDrosophila ProteinsEmbryo, NonmammalianHomeodomain ProteinsProcessing BodiesRNA, Messenger, StoredTrans-ActivatorsDEAD-box RNA HelicasesDrosophila ProteinsHomeodomain ProteinsRNA, Messenger, StoredTrans-Activatorsaxis patterningbiomolecular condensatesDrosophilaintrinsically disordered regionsin vivo imagingmRNA regulationphase separationprocessing bodiesribonucleoprotein

Identifiers

PMID34655524
PMCPMC8555633
OpenAlexW3201780883

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.