ArticleThe New phytologist2022
Dissecting the labdane-related diterpenoid biosynthetic gene clusters in rice reveals directional cross-cluster phytotoxicity.
Article in The New phytologist, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Divergent roles of ent-kaurene oxidase paralogs in rice momilactone biosynthesis.Journal of integrative plant biology · 2026Article
- The role of isoprenoids in the chemical interaction between plants and other organisms in their rhizosphere.aBIOTECH · 2025Review
- Large-scale genomic deletion inFrontiers in plant science · 2025Article
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
- Enhancing Crop Resilience: Insights from Labdane-Related Diterpenoid Phytoalexin Research in Rice (Current issues in molecular biology · 2024Review
- Double-barreled defense: dualaBIOTECH · 2024Article
- A century of studying plant secondary metabolism-From "what?" to "where, how, and why?"Plant physiology · 2024Review
- Isolation and identification of allelochemicals and their activities and functions.Journal of pesticide science · 2024Article
- PnMYB4 negatively modulates saponin biosynthesis inHorticulture research · 2023Article
- Defensive Molecules Momilactones A and B: Function, Biosynthesis, Induction and Occurrence.Toxins · 2023Review
- Article
- Plant (di)terpenoid evolution: from pigments to hormones and beyond.Natural product reports · 2023Review
- Rice Phytoalexins: Half a Century of Amazing Discoveries; Part I: Distribution, Biosynthesis, Chemical Synthesis, and Biological Activities.Plants (Basel, Switzerland) · 2023Review
- Functional diversity and metabolic engineering of plant-specialized metabolites.Life metabolism · 2022Review
- Plant biosynthetic gene clusters in the context of metabolic evolution.Natural product reports · 2022Review
- Deletion of Diterpenoid Biosynthetic GenesInternational journal of molecular sciences · 2022Article
- Hrip1 mediates rice cell wall fortification and phytoalexins elicitation to confer immunity againstFrontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Rice (Oryza sativa) is a staple food crop and serves as a model cereal plant. It contains two biosynthetic gene clusters (BGCs) for the production of labdane-related diterpenoids (LRDs), which serve important roles in combating biotic and abiotic stress. While plant BGCs have been subject to genetic analyses, these analyses have been largely confined to the investigation of single genes. CRISPR/Cas9-mediated genome editing was used to precisely remove each of these BGCs, as well as simultaneously knock out both BGCs. Deletion of the BGC from chromosome 2 (c2BGC), which is associated with phytocassane biosynthesis, but not that from chromosome 4 (c4BGC), which is associated with momilactone biosynthesis, led to a lesion mimic phenotype. This phenotype is dependent on two closely related genes encoding cytochrome P450 (CYP) mono-oxygenases, CYP76M7 and CYP76M8, from the c2BGC. However, rather than being redundant, CYP76M7 has been associated with the production of phytocassanes, whereas CYP76M8 is associated with momilactone biosynthesis. Intriguingly, the lesion mimic phenotype is not present in a line with both BGCs deleted. These results reveal directional cross-cluster phytotoxicity, presumably arising from the accumulation of LRD intermediates dependent on the c4BGC in the absence of CYP76M7 and CYP76M8, further highlighting their interdependent evolution and the selective pressures driving BGC assembly.
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