ArticleBiomedical microdevices2021
Dynamic flow and shear stress as key parameters for intestinal cells morphology and polarization in an organ-on-a-chip model.
Article in Biomedical microdevices, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 2 of them syntheses that pooled it.
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Who cites it
42 citing papers in PubMed, 2 syntheses or guidelines pooled it, 71 citations in OpenAlex.
- Organ-on-a-Chip Devices to Simulate the Dentin-Pulp Complex: A Qualitative Systematic Review.Annals of biomedical engineering · 2026Pooled it
- A quantitative meta-analysis comparing cell models in perfused organ on a chip with static cell cultures.Scientific reports · 2023Pooled it
- Mechanics of small intestine motility for oral macromolecular delivery: modelling segmentation versus peristalsis.Drug delivery · 2026Article
- Systematic standardization of organ-on-a-chip under controlled flow conditions: Use case with Caki-1 and A549 cell lines.MethodsX · 2026Article
- A Practical Gut-On-Chip Workflow for Imaging-Based Assessment of Epithelial Barrier Dynamics During E. coli Co-Culture.Biotechnology and bioengineering · 2026Article
- Biomimetic villi-crypt scaffold-on-chip with tunable mechanical properties for intestinal epithelium modeling.Materials today. Bio · 2026Article
- Use of organ-on-chip devices and single particle ICP-MS for assessing dynamic in vitro bioavailability of silver and titanium dioxide nanoparticles from foodstuff.Mikrochimica acta · 2026Article
- Modeling Clostridioides difficile toxin pathogenesis and antiserum protection in an immunocompetent intestine-on-chip platform.Scientific reports · 2026Article
- Advanced technologies of artery-on-a-chip: a review of construction strategies and disease models.Angiogenesis · 2026Review
- A 3D-Printed Pump-Free Multi-Organ-on-a-Chip Platform for Modeling the Intestine-Liver-Muscle Axis.Micromachines · 2026Article
- Mechanomedicine in digestive surgery: a theranostic framework integrating mechanical diagnostics and therapeutic modulation across the perioperative continuum.Theranostics · 2026Review
- Transcriptomic profiling reveals substrate- and shear stress-dependent maturation of human small intestinal epithelial cells.Frontiers in pharmacology · 2026Article
- Construction of Vascularized Intestinal Organoids Based on Scaffolds, Hydrogels, and 3D Printing Technologies and Their Applications in Drug Delivery.International journal of nanomedicine · 2026Review
- Review
- In Vitro and Ex Vivo Models to Study Molecular Trafficking Across the Human Intestinal Barrier.International journal of molecular sciences · 2025Review
- Half-Pipe Melt Electrowritten Scaffolds Support Engineering of an Immunocompetent Hydrogel-Embedded Intestine-on-a-Chip.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The Fluidic Shear Stress Loading Method Enables Mechanobiological Stimulation in an On-Chip Pump-Integrated Microphysiological System.Micromachines · 2025Article
- Flow-induced mechano-modulation of intestinal permeability on chip.Materials today. Bio · 2025Article
- Small Intestine on a Chip Demonstrates Physiologic Mucus Secretion in the Presence of Lacticaseibacillus rhamnosus Biofilm.Biotechnology and bioengineering · 2025Article
- Biomimetic culture substrates for modelling homeostatic intestinal epithelium in vitro.Nature communications · 2025Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gut-on-a-chip microfluidic devices have emerged as versatile and practical systems for modeling the human intestine in vitro. Cells cultured under microfluidic conditions experience the effect of shear stress, used as a biomechanical cue to promote a faster cell polarization in Caco-2 cells when compared with static culture conditions. However, published systems to date have utilized a constant flow rate that fails to account for changes in cell shear stress ([Formula: see text]) resulting from changes in cell elongation that occur with differentiation. In this study, computational fluid dynamics (CFD) simulations predict that cells with villi-like morphology experience a [Formula: see text] higher than bulge-like cells at the initial growth stages. Therefore, we investigated the use of a dynamic flow rate to maintain a constant [Formula: see text] across the experiment. Microscopic assessment of cell morphology and dome formation confirmed the initiation of Caco-2 polarization within three days. Next, adopting our dynamic approach, we evaluated whether the following decreased flow could still contribute to complete cell differentiation if compared with the standard constant flow methodology. Caco-2 cells polarized under both conditions, secreted mucin-2 and villin and formed tight junctions and crypt-villi structures. Gene expression was not impacted using the dynamic flow rate. In conclusion, our dynamic flow approach still facilitates cell differentiation while enabling a reduced consumption of reagents.
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