Evidence map›Paper›PMID 34655020›Full record

ArticleMolecular biology reports2021

Transcriptome-derived microsatellite markers for population diversity analysis in Archidendron clypearia (Jack) I.C. Nielsen.

Dandan Li, Mei Li, Fagen Li, Qijie Weng, Changpin Zhou, Shineng Huang, Siming Gan

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Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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3 · Its place in the literature

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0 citing papers in PubMed, 2 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Dandan Li *State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Road, Beijing, 100091, China.
Mei Li *Key Laboratory of National Forestry and Grassland Administration on Tropical Forestry Research, Research Institute of Tropical Forestry, Chinese Academy of Forestry, 682 Guangshan Yi Road, Guangzhou, 510520, Guangdong, China.
Fagen LiKey Laboratory of National Forestry and Grassland Administration on Tropical Forestry Research, Research Institute of Tropical Forestry, Chinese Academy of Forestry, 682 Guangshan Yi Road, Guangzhou, 510520, Guangdong, China.
Qijie WengKey Laboratory of National Forestry and Grassland Administration on Tropical Forestry Research, Research Institute of Tropical Forestry, Chinese Academy of Forestry, 682 Guangshan Yi Road, Guangzhou, 510520, Guangdong, China.
Changpin ZhouKey Laboratory of National Forestry and Grassland Administration on Tropical Forestry Research, Research Institute of Tropical Forestry, Chinese Academy of Forestry, 682 Guangshan Yi Road, Guangzhou, 510520, Guangdong, China.
Shineng HuangKey Laboratory of National Forestry and Grassland Administration on Tropical Forestry Research, Research Institute of Tropical Forestry, Chinese Academy of Forestry, 682 Guangshan Yi Road, Guangzhou, 510520, Guangdong, China.
Siming GanState Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Road, Beijing, 100091, China. siminggan@caf.ac.cn.ORCID http://orcid.org/0000-0001-6677-9860
Research Institute of Tropical Forestry · CN

Funding

Fundamental Research Funds of Chinese Academy of Forestry CAFYBB2018SY019Public Welfare Research and Capacity Building Funds for Social Development from the Department of Science and Technology of Guangdong Province 2017A020213002
6 · The paper itself

Abstract

backgroundThe medicinal woody leguminous genus Archidendron F. Mueller serves as important herbal resources for curing upper respiratory tract infection, acute pharyngitis, tonsillitis, and gastroenteritis. However, genomic resources including transcriptomic sequences and molecular markers remain scarce in the genus. METHODS AND

resultsTranscriptome sequencing, genic microsatellite marker development, and population diversity analysis were conducted in Archidendron clypearia (Jack) I.C. Nielsen. Flower and flower bud transcriptomes were de novo assembled into 173,172 transcripts, with an average transcript length of 1597.3 bp and an N50 length of 2427 bp. A total of 34,701 microsatellite loci were identified from 26,716 (15.4 %) transcripts. Primer pairs were designed for 718 microsatellite loci, of which 456 (63.5 %) were polymorphic. Of the 456 polymorphic markers, 391 (85.7 %) and 402 (88.1 %) were transferable to A. lucidum (Benth.) I.C. Nielsen and A. multifoliolatum (H.Q. Wen) T.L. Wu, respectively. Using a subset of 15 microsatellite markers, relatively high genetic diversity was detected over two A. clypearia populations, with overall mean expected heterozygosity (H

conclusionsThis study represents the first attempt to conduct transcriptome sequencing, SSR marker development, and population genetics analysis in the medicinally important genus Archidendron. Our results will offer valuable resources and information for further genetic studies and practical applications in Archidendron and the related taxa.

Indexed as

Expressed Sequence TagsFabaceaeFlowersGene ExpressionGene Expression ProfilingGene Expression Regulation, PlantGenetics, PopulationGenetic VariationHybridization, GeneticMicrosatellite RepeatsPolymorphism, GeneticTranscriptomeArchidendron clypearia (Jack) I.C. NielsenGenetic diversityGenic microsatellite markerMedicinal treeTranscriptome

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PMID34655020
OpenAlexW3174002961

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