ArticleThe ISME journal2022
Adaptation to an amoeba host drives selection of virulence-associated traits in Vibrio cholerae.
Article in The ISME journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Genomic and phenotypic diversification ofMicrobiology spectrum · 2026Article
- Type VI secretion systems at the interface between bacteria and eukaryotic cells.Genetics and molecular biology · 2026Article
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- Diet modulates Vibrio cholerae colonization and competitive outcomes with the gut microbiota.Cell host & microbe · 2025Article
- Article
- Temporal and Spatial Survey on the Abundance of Amoebae and Bacteria in an Estuary and the Role of Environmental Parameters.Environmental microbiology reports · 2025Article
- Paramoeba atlantica as a Natural Intracellular Niche for Vibrios in Marine Ecosystems.Environmental microbiology · 2025Article
- Stress adaptation underMicrobiology spectrum · 2025Article
- A new understanding of Acanthamoeba castellanii: dispelling the role of bacterial pore-forming toxins in cyst formation and amoebicidal actions.Cell death discovery · 2025Article
- Tetrahymena predation drives adaptive evolution of Salmonella by disrupting O-antigen biosynthesis and upregulating transcriptional regulator csgD.The ISME journal · 2025Article
- A socioenvironmental approach to the nosogenic potential of freshwaters with presence of thermotolerant free-living amoebae in Costa Rica.Frontiers in public health · 2025Article
- Growth response ofIranian journal of microbiology · 2024Article
- Review
- Making a Pathogen? Evaluating the Impact of Protist Predation on the Evolution of Virulence in Serratia marcescens.Genome biology and evolution · 2024Article
- Phylogenomic, structural, and cell biological analyses reveal thatMicrobiology spectrum · 2024Article
- The role of naturally acquired intracellular Pseudomonas aeruginosa in the development of Acanthamoeba keratitis in an animal model.PLoS neglected tropical diseases · 2024Article
- Zooming in on the intracellular microbiome composition of bacterivorousISME communications · 2024Article
- Increased iron utilization and oxidative stress tolerance in aApplied and environmental microbiology · 2023Article
- Phagotrophic protists preserve antibiotic-resistant opportunistic human pathogens in the vegetable phyllosphere.ISME communications · 2023Article
- Homeostasis of cellular amino acids in Acanthamoeba castellanii exposed to different media under amoeba-bacteria coculture conditions.BMC microbiology · 2023Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Predation by heterotrophic protists drives the emergence of adaptive traits in bacteria, and often these traits lead to altered interactions with hosts and persistence in the environment. Here we studied adaptation of the cholera pathogen, Vibrio cholerae during long-term co-incubation with the protist host, Acanthamoeba castellanii. We determined phenotypic and genotypic changes associated with long-term intra-amoebal host adaptation and how this impacts pathogen survival and fitness. We showed that adaptation to the amoeba host leads to temporal changes in multiple phenotypic traits in V. cholerae that facilitate increased survival and competitive fitness in amoeba. Genome sequencing and mutational analysis revealed that these altered lifestyles were linked to non-synonymous mutations in conserved regions of the flagellar transcriptional regulator, flrA. Additionally, the mutations resulted in enhanced colonisation in zebrafish, establishing a link between adaptation of V. cholerae to amoeba predation and enhanced environmental persistence. Our results show that pressure imposed by amoeba on V. cholerae selects for flrA mutations that serves as a key driver for adaptation. Importantly, this study provides evidence that adaptive traits that evolve in pathogens in response to environmental predatory pressure impact the colonisation of eukaryotic organisms by these pathogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.