Evidence map›Paper›PMID 34654824›Full record

ArticleScientific data2021

Systematic phenotyping and characterization of the 5xFAD mouse model of Alzheimer's disease.

Stefania Forner, Shimako Kawauchi, Gabriela Balderrama-Gutierrez, Enikö A Kramár, Dina P Matheos, Jimmy Phan, Dominic I Javonillo, Kristine M Tran, Edna Hingco, Celia da Cunha and 11 more

Abstract readDataset
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific data, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 317 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
317citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

317 citing papers in PubMed, 2 syntheses or guidelines pooled it.

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  6. Diffusion MRI reveals amyloid plaque-associated cellular signatures through spatial transcriptomic integration.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2026
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257 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Stefania Forner *Institute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA.ORCID 0000-0001-7977-4866
Shimako Kawauchi *Transgenic Mouse Facility, University Laboratory Animal Resources, Office of Research, University of California, Irvine, CA, 92697, USA.
Gabriela Balderrama-GutierrezDepartment of Developmental and Cell Biology, University of California, Irvine, CA, 92697, USA.
Enikö A KramárDepartment of Neurobiology and Behavior, University of California, Irvine, CA, 92697, USA.
Dina P MatheosDepartment of Neurobiology and Behavior, University of California, Irvine, CA, 92697, USA.
Jimmy PhanInstitute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA.
Dominic I JavonilloInstitute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA.
Kristine M TranInstitute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA.
Edna HingcoInstitute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA.
Celia da CunhaInstitute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA.
Narges RezaieDepartment of Developmental and Cell Biology, University of California, Irvine, CA, 92697, USA.ORCID 0000-0001-9326-9954
Joshua A AlcantaraTransgenic Mouse Facility, University Laboratory Animal Resources, Office of Research, University of California, Irvine, CA, 92697, USA.
David Baglietto-VargasInstitute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA.
Camden JansenDepartment of Developmental and Cell Biology, University of California, Irvine, CA, 92697, USA.
Jonathan NeumannTransgenic Mouse Facility, University Laboratory Animal Resources, Office of Research, University of California, Irvine, CA, 92697, USA.
Marcelo A WoodDepartment of Neurobiology and Behavior, University of California, Irvine, CA, 92697, USA.
Grant R MacGregorTransgenic Mouse Facility, University Laboratory Animal Resources, Office of Research, University of California, Irvine, CA, 92697, USA.ORCID 0000-0001-7598-9501
Ali MortazaviDepartment of Developmental and Cell Biology, University of California, Irvine, CA, 92697, USA.ORCID 0000-0002-4259-6362
Andrea J TennerInstitute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA.ORCID 0000-0003-3000-024X
Frank M LaFerlaInstitute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA.ORCID 0000-0003-2324-6911
Kim N GreenInstitute for Memory Impairments and Neurological Disorders (UCI MIND), University of California, Irvine, CA, 92697, USA. kngreen@uci.edu.

Funding

The IU/JAX Alzheimer's Disease Precision Models Center: Rat F344U54AG054345 · NIA · INDIANA UNIVERSITY INDIANAPOLIS · PI Gareth R Howell · 2016 to 2026
$95.6M
UC Irvine MODEL-ADU54AG054349 · NIA · UNIVERSITY OF CALIFORNIA-IRVINE · PI Craig E Stark · 2017 to 2026
$71.9M
Systems Genetics Approach to Determine Interactors of Apolipoprotein E in Alzheimer’s DiseaseRF1AG055104 · NIA · JACKSON LABORATORY · PI CARTER, GREGORY W, HOWELL, GARETH R · 2018 to 2018
$4.1M
Microglia are necessary for cortical plaque formation in Alzheimer's diseaseRF1AG056768 · NIA · UNIVERSITY OF CALIFORNIA-IRVINE · PI GREEN, KIM · 2018 to 2018
$2.7M
NIA NIH HHS RF1 AG055104NIA NIH HHS RF1 AG056768NIA NIH HHS U54 AG054345NIA NIH HHS U54 AG054349
6 · The paper itself

Abstract

Mouse models of human diseases are invaluable tools for studying pathogenic mechanisms and testing interventions and therapeutics. For disorders such as Alzheimer's disease in which numerous models are being generated, a challenging first step is to identify the most appropriate model and age to effectively evaluate new therapeutic approaches. Here we conducted a detailed phenotypic characterization of the 5xFAD model on a congenic C57BL/6 J strain background, across its lifespan - including a seldomly analyzed 18-month old time point to provide temporally correlated phenotyping of this model and a template for characterization of new models of LOAD as they are generated. This comprehensive analysis included quantification of plaque burden, Aβ biochemical levels, and neuropathology, neurophysiological measurements and behavioral and cognitive assessments, and evaluation of microglia, astrocytes, and neurons. Analysis of transcriptional changes was conducted using bulk-tissue generated RNA-seq data from microdissected cortices and hippocampi as a function of aging, which can be explored at the MODEL-AD Explorer and AD Knowledge Portal. This deep-phenotyping pipeline identified novel aspects of age-related pathology in the 5xFAD model.

Indexed as

Disease Models, AnimalPhenotypeAlzheimer DiseaseAnimalsBehavior, AnimalHippocampusLong-Term PotentiationMiceMice, Inbred C57BLRNA-SeqSynaptic Transmission

Identifiers

PMID34654824
PMCPMC8519958

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.