Evidence map›Paper›PMID 34652441›Full record

ArticleJournal of biochemistry2022

Long non-coding RNA AFAP1-AS1 promotes thyroid cancer progression by sponging miR-204-3p and upregulating DUSP4.

Hongzhi Ma, Qian Shi, Jugao Fang, Ru Wang, Jianyu Zhao, Sitong Lin, Jiajing Dong, Yan Zhang, Xixi Shen, Jiaming Chen and 1 more

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Journal of biochemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Hongzhi MaDepartment of Otorhinolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Chinese Medical University, Beijing 100730, China.
Qian ShiDepartment of Otorhinolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Chinese Medical University, Beijing 100730, China.
Jugao FangDepartment of Otorhinolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Chinese Medical University, Beijing 100730, China.
Ru WangDepartment of Otorhinolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Chinese Medical University, Beijing 100730, China.
Jianyu ZhaoDepartment of Endocrinology, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, China.
Sitong LinDepartment of Endocrinology, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, China.
Jiajing DongDepartment of Endocrinology, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, China.
Yan ZhangDepartment of Endocrinology, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, China.
Xixi ShenDepartment of Otorhinolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Chinese Medical University, Beijing 100730, China.
Jiaming ChenDepartment of Otorhinolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Chinese Medical University, Beijing 100730, China.
Qi ZhongDepartment of Otorhinolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Chinese Medical University, Beijing 100730, China.
Beijing Tongren Hospital · CNJilin University · CNUnion Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Long non-coding RNA actin filament-associated protein 1-antisense RNA 1 (AFAP1-AS1) shows crucial regulatory function in tumor progression. Nonetheless, the biological function and underlying mechanism of AFAP1-AS1 in the progression of thyroid cancer is still unclear. Expressions of AFAP1-AS1, miR-204-3p and DUSP4 were quantified utilizing quantitative real-time polymerase chain reaction and/or western blot. In loss-of-function and gain-of-function assays, cell proliferation, migration and invasion were appraised by CCK-8 assay, wound healing assay, Transwell migration and invasion assays, respectively. Luciferase reporter assay was employed for validating the interaction between miR-204-3p and AFAP1-AS1 or the 3'UTR of dual specificity phosphatase 4 (DUSP4). AFAP1-AS1 was highly expressed in thyroid cancer tissues and cell lines. Highly expressed AFAP1-AS1 was in association with advanced TNM stage and positive lymph node metastasis. Knockdown of AFAP1-AS1 suppressed the proliferation, migration and invasion of thyroid cancer cells, and overexpression of AFAP1-AS1 induced a reversed effect. MiR-204-3p was targetedly repressed by AFAP1-AS1, and miR-204-3p could negatively regulate DUSP4 expression. AFAP1-AS1 augmented the expression of DUSP4 via repressing miR-204-3p, and the effects of AFAP1-AS1 overexpression on thyroid cancer cells were also partly abolished by miR-204-3p restoration. In summary, AFAP1-AS1 facilitates thyroid cancer cell proliferation, migration and invasion by regulating miR-204-3p/DUSP4 axis.

Indexed as

MicroRNAsRNA, Long NoncodingThyroid NeoplasmsCell Line, TumorCell MovementCell ProliferationDual-Specificity PhosphatasesGene Expression Regulation, NeoplasticHumansMitogen-Activated Protein Kinase PhosphatasesAFAP1-AS1 long noncoding RNA, humanDual-Specificity PhosphatasesDUSP4 protein, humanMicroRNAsMIRN204 microRNA, humanMitogen-Activated Protein Kinase PhosphatasesRNA, Long NoncodingAFAP1-AS1DUSP4metastasisMiR-204-3pproliferationthyroid cancer

Identifiers

PMID34652441
OpenAlexW3207695424

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.