ArticlePsychopharmacology2022
The zebrafish (Danio rerio) anxiety test battery: comparison of behavioral responses in the novel tank diving and light-dark tasks following exposure to anxiogenic and anxiolytic compounds.
Article in Psychopharmacology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
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Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it, 81 citations in OpenAlex.
- Anxiolytic-like effects of acute serotonin-releasing agents in zebrafish models of anxiety: experimental study and systematic review.Acta neuropsychiatrica · 2024Pooled it
- Arbutin mediated neuroprotection in zebrafish model of traumatic brain injury via modulating Nrf2/NF-κB pathway.Inflammopharmacology · 2026Article
- Photoperiod driven modulation of behavior, gut microbiota, and brain transcriptomics in zebrafish.Pflugers Archiv : European journal of physiology · 2026Article
- Twenty-four-hour post-anaesthetic behavioural effects of menthol and thymol compared to conventional anaesthetics in zebrafish.Fish physiology and biochemistry · 2026Article
- GBR 12909, a Selective Dopamine Transporter Inhibitor, Differentially Modulates Affective, Exploratory and Cognitive Domains in Zebrafish.The European journal of neuroscience · 2026Article
- Neurobehavioral Effects of Two Distinct Anti-Inflammatory Drugs in a Two-Week Chronic Stress Model in Zebrafish.Brain sciences · 2026Article
- Sex-specific neurological dysregulation may underpin distinctly different male and female behavioural phenotypes in a zebrafish model of autism spectrum disorder.Behavioral and brain functions : BBF · 2026Article
- Natural products modulate social behavior and gut microbiota in a valproic acid-induced zebrafish model of autism spectrum disorder.Biomolecules & biomedicine · 2026Article
- Antioxidant, Anti-Cholinesterase, and Neuroprotective Properties ofAntioxidants (Basel, Switzerland) · 2026Article
- Impact of dietary composition on behavioural expression and gut microbiome dynamics in zebrafish.Pflugers Archiv : European journal of physiology · 2025Article
- Comparative evaluation of MPTP and rotenone as inducing agents for Parkinson's disease in adult zebrafish: Behavioural and histopathological insights.Toxicology reports · 2025Article
- Distinct Swimming Behavioral Phenotypes Following Serotonin and Dopamine Transporter Modulation in the Adult Zebrafish Novel Tank Diving Test (NTT).Pharmaceuticals (Basel, Switzerland) · 2025Article
- Ethanol-Withanolides Interactions: Compound-Specific Effects on Zebrafish Larvae Locomotor Behavior and GABAA Receptor Subunit Expression.International journal of molecular sciences · 2025Article
- Inhibition mediated by group III metabotropic glutamate receptors regulates habenula activity and defensive behaviors.Nature communications · 2025Article
- Effects of Environmental Temperature Variation on the Spatio-Temporal Shoaling Behaviour of Adult Zebrafish (Animals : an open access journal from MDPI · 2025Article
- Individual Differences in Boldness Influence Working Memory and Stress-Induced Repetitive Behaviors in Zebrafish.The European journal of neuroscience · 2025Article
- Housing and husbandry factors affecting zebrafish novel tank test responses: a global multi-laboratory study.Lab animal · 2025Article
- The Impact of Oxytocin on Stimulus Discrimination of Zebrafish Albino and Non-Albino Models.International journal of molecular sciences · 2025Article
- Naltrexone Alters Neurochemical and Behavioral Parameters in a Zebrafish Model of Repeated Alcohol Exposure.Neurochemical research · 2025Article
- Neuroprotective Potential ofBiomolecules · 2025Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
rationaleTriangulation of approaches (i.e., using several tests of the same construct) can be extremely useful for increasing the robustness of the findings being widely used when working with behavioral testing, especially when using rodents as a translational model. Although zebrafish are widely used in neuropharmacology research due to their high-throughput screening potential for new therapeutic drugs, behavioral test battery effects following pharmacological manipulations are still unknown.
methodsHere, we tested the effects of an anxiety test battery and test time following pharmacological manipulations in zebrafish by using two behavioral tasks: the novel tank diving task (NTT) and the light-dark test (LDT). Fluoxetine and conspecific alarm substance (CAS) were chosen to induce anxiolytic and anxiogenic-like behavior, respectively.
resultsFor non-drug-treated animals, no differences were observed for testing order (NTT → LDT or LDT → NTT) and there was a strong correlation between performances on the two behavioral tasks. However, we found that during drug treatment, NTT/LDT responses are affected by the tested order depending on the test time being fluoxetine effects higher at the second behavioral task (6 min later) and CAS effects lower across time.
conclusionsOverall, our data supports the use of baseline behavior assessment using this anxiety test battery. However, when working with drug exposure, data analysis must carefully consider time-drug-response and data variability across behavioral tasks.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.