Evidence map›Paper›PMID 34651104›Full record

ArticleACS pharmacology & translational science2021

Inhibition of Severe Acute Respiratory Syndrome Coronavirus 2 Replication by Hypertonic Saline Solution in Lung and Kidney Epithelial Cells.

Rafael R G Machado, Talita Glaser, Danielle B Araujo, Lyvia Lintzmaier Petiz, Danielle B L Oliveira, Giuliana S Durigon, Alessandra L Leal, João Renato R Pinho, Luis C S Ferreira, Henning Ulrich and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS pharmacology & translational science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.5field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 25 citations in OpenAlex.

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  7. Efficacy of BREATHOXJournal of clinical medicine · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Rafael R G MachadoDepartment of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508, Brazil.
Talita GlaserDepartment of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo 05508, Brazil.
Danielle B AraujoDepartment of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508, Brazil.
Lyvia Lintzmaier PetizDepartment of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo 05508, Brazil.
Danielle B L OliveiraDepartment of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508, Brazil.
Giuliana S DurigonMedical School Clinical Hospital, University of São Paulo, São Paulo 05508, Brazil.
Alessandra L LealGaspar Vianna Clinic Hospital Foundation, Belém 66083, Brazil.
João Renato R PinhoHospital Israelita Albert Einstein, São Paulo 05652, Brazil.
Luis C S FerreiraDepartment of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508, Brazil.
Henning UlrichDepartment of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo 05508, Brazil.ORCID https://orcid.org/0000-0002-2114-3815
Edison L DurigonDepartment of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508, Brazil.
Cristiane Rodrigues GuzzoDepartment of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo 05508, Brazil.ORCID https://orcid.org/0000-0002-5664-8055
Universidade de São Paulo · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An unprecedented global health crisis has been caused by a new virus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We performed experiments to test if a hypertonic saline solution was capable of inhibiting virus replication. Our data show that 1.2% NaCl inhibited virus replication by 90%, achieving 100% of inhibition at 1.5% in the nonhuman primate kidney cell line Vero, and 1.1% of NaCl was sufficient to inhibit the virus replication by 88% in human epithelial lung cell line Calu-3. Furthermore, our results indicate that the inhibition is due to an intracellular mechanism and not to the dissociation of the spike SARS-CoV-2 protein and its human receptor. NaCl depolarizes the plasma membrane causing a low energy state (high ADP/ATP concentration ratio) without impairing mitochondrial function, supposedly associated with the inhibition of the SARS-CoV-2 life cycle. Membrane depolarization and intracellular energy deprivation are possible mechanisms by which the hypertonic saline solution efficiently prevents virus replication

Identifiers

PMID34651104
PMCPMC8442612
OpenAlexW3197084554

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.