Evidence map›Paper›PMID 34651022›Full record

ArticleFrontiers in cardiovascular medicine2021

Notch Intracellular Domain Plasmid Delivery via Poly(Lactic-Co-Glycolic Acid) Nanoparticles to Upregulate Notch Pathway Molecules.

Victoria L Messerschmidt, Uday Chintapula, Aneetta E Kuriakose, Samantha Laboy, Thuy Thi Dang Truong, LeNaiya A Kydd, Justyn Jaworski, Zui Pan, Hashem Sadek, Kytai T Nguyen and 1 more

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In one paragraph

Article in Frontiers in cardiovascular medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Frontiers in physiology · 2022
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Victoria L MesserschmidtDepartment of Bioengineering, University of Texas at Arlington, Arlington, TX, United States.
Uday ChintapulaDepartment of Bioengineering, University of Texas at Arlington, Arlington, TX, United States.
Aneetta E KuriakoseDepartment of Bioengineering, University of Texas at Arlington, Arlington, TX, United States.
Samantha LaboyDepartment of Bioengineering, University of Texas at Arlington, Arlington, TX, United States.
Thuy Thi Dang TruongDepartment of Bioengineering, University of Texas at Arlington, Arlington, TX, United States.
LeNaiya A KyddDepartment of Bioengineering, University of Texas at Arlington, Arlington, TX, United States.
Justyn JaworskiDepartment of Bioengineering, University of Texas at Arlington, Arlington, TX, United States.
Zui PanCollege of Nursing and Health Innovation, University of Texas at Arlington, Arlington, TX, United States.
Hashem SadekDepartment of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX, United States.
Kytai T NguyenDepartment of Bioengineering, University of Texas at Arlington, Arlington, TX, United States.
Juhyun LeeDepartment of Bioengineering, University of Texas at Arlington, Arlington, TX, United States.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Notch signaling is a highly conserved signaling system that is required for embryonic development and regeneration of organs. When the signal is lost, maldevelopment occurs and leads to a lethal state. Delivering exogenous genetic materials encoding Notch into cells can reestablish downstream signaling and rescue cellular functions. In this study, we utilized the negatively charged and FDA approved polymer poly(lactic-co-glycolic acid) to encapsulate Notch Intracellular Domain-containing plasmid in nanoparticles. We show that primary human umbilical vein endothelial cells (HUVECs) readily uptake the nanoparticles with and without specific antibody targets. We demonstrated that our nanoparticles are non-toxic, stable over time, and compatible with blood. We further demonstrated that HUVECs could be successfully transfected with these nanoparticles in static and dynamic environments. Lastly, we elucidated that these nanoparticles could upregulate the downstream genes of Notch signaling, indicating that the payload was viable and successfully altered the genetic downstream effects.

Indexed as

gene deliverynanoparticlesnon-viral transfectionNotch signalingPLGA

Identifiers

PMID34651022
PMCPMC8507495

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.