ArticleAmerican journal of translational research2021
CircRNA_002178 as a ceRNA promotes the development of colorectal cancer by regulating miR-542-3p/CREB1.
Article in American journal of translational research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed, 4 citations in OpenAlex.
- Circ_0000370 Plays an Oncogenic Role in Colorectal Cancer by Regulating the miR-502-5p/SIRT1 Axis.Biochemical genetics · 2024Article
- Circular RNAs as Diagnostic and Prognostic Indicators of Colorectal Cancer: A Pooled Analysis of Individual Studies.Pathology oncology research : POR · 2022Article
- Integrated analysis of the functions and clinical implications of exosome circRNAs in colorectal cancer.Frontiers in immunology · 2022Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveColorectal cancer (CRC) is a malignant tumor commonly found in the digestive tract. This study aimed to explore the effect of circRNA_002178 as a competing endogenous RNA in the development of CRC by regulating the miR-542-3p/cAMP response element binding protein 1 (CREB1) axis and its molecular mechanism.
methodsThe relative expressions of circ_002178, miR-542-3p, and CREB1 in patients' cell lines and CRC tissues were measured using Western blot and qRT-PCR. The localization and expression of circ_002178 were determined using fluorescence in situ hybridization and nucleocytoplasmic separation tests. The targeting relationships among circ_002178, miR-542-3p, and CREB1 were validated using RNA immunoprecipitation and dual luciferase reporter assays. The cells' proliferation, invasion, and colony forming ability were tested using CCK8, Transwell, and Clone formation assays, respectively. The cellular glucose consumption, lactification, and adenosine triphosphate (ATP) production were measured using glucose uptake colorimetric assay kits, lactate colorimetric assay kits and ATP assay kits, respectively.
resultsThe circ_002178 and CREB1 expressions were up-regulated in the CRC cells and tissues, and the miR-542-3p expression was down-regulated (all P<0.05). The circ_002178 knockdown inhibited the proliferation, invasion, colony formation, and glycolysis of the CRC cells
conclusioncirc_002178 promotes the invasion, proliferation, colony formation, and glycolysis of CRC cells by regulating the miR-542-3p/CREB1 axis, thus driving the development of CRC.
Indexed as
Identifiers
34650680PMC8507087W3205704081What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.