ArticleScience (New York, N.Y.)2021
Mechanism of siRNA production by a plant Dicer-RNA complex in dicing-competent conformation.
Article in Science (New York, N.Y.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
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Who cites it
62 citing papers in PubMed.
- Multiple Argonaute complexes orchestrate epigenetic silencing in the Arabidopsis germline.Nature communications · 2026Article
- Nanoparticle-enhanced multi-target RNAi in Myzus persicae: Molecular mechanisms, cross-kingdom sRNA transfer, and efficacy for sustainable pest management.Pest management science · 2026Article
- RNA regulation in plants.Science China. Life sciences · 2026Review
- Article
- A spontaneous termination mechanism of RNA polymerase V shapes the DNA methylation landscape in plants.The EMBO journal · 2026Article
- Molecular basis of DRB4-assisted long RNA processing and 21-nucleotide siRNA biogenesis by DCL4 in plants.Nature plants · 2026Article
- Molecular basis of plant DCL4 action that outcompetes DCL2.Nature plants · 2026Article
- Transcriptome-wide analysis of Arabidopsis DICER-LIKE1 RNA substrates.Nucleic acids research · 2026Article
- Molecular Identification and RNA-Based Management of Fungal Plant Pathogens: From PCR to CRISPR/Cas9.International journal of molecular sciences · 2026Review
- SHH1 cooperates with the DNA methylation reader MBD7 to suppress transcriptional silencing of promoter-methylated genes in Arabidopsis.Plant communications · 2026Article
- Genome-wide identification and characterization ofFrontiers in plant science · 2026Article
- Plant microRNA maturation and function.Nature reviews. Molecular cell biology · 2026Review
- DNA Methylation in Rice: Mechanisms, Regulatory Roles, and Beyond.International journal of molecular sciences · 2025Review
- Role of ncRNAs in the Development of Chronic Pain.Non-coding RNA · 2025Review
- Molecular Mechanisms Underlying the Establishment, Maintenance, and Removal of DNA Methylation in Plants.Annual review of plant biology · 2025Review
- The biogenesis and regulation of animal microRNAs.Nature reviews. Molecular cell biology · 2025Review
- A new level of RNA-based plant protection: dsRNAs designed from functionally characterized siRNAs highly effective against Cucumber mosaic virus.Nucleic acids research · 2025Article
- A double-edged sword in antiviral defence: ATG7 binding dicer to promote virus replication.Cellular and molecular life sciences : CMLS · 2025Article
- Small Interfering RNAs as Critical Regulators of Plant Life Process: New Perspectives on Regulating the Transcriptomic Machinery.International journal of molecular sciences · 2025Review
- Structural basis of endo-siRNA processing by Drosophila Dicer-2 and Loqs-PD.Nucleic acids research · 2025Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors.
Funding
Abstract
In eukaryotes, small RNAs (sRNAs) play critical roles in multiple biological processes. Dicer endonucleases are a central part of sRNA biogenesis. In plants, DICER-LIKE PROTEIN 3 (DCL3) produces 24-nucleotide (nt) small interfering RNAs (siRNAs) that determine the specificity of the RNA-directed DNA methylation pathway. Here, we determined the structure of a DCL3–pre-siRNA complex in an active dicing-competent state. The 5′-phosphorylated A1 of the guide strand and the 1-nt 3′ overhang of the complementary strand are specifically recognized by a positively charged pocket and an aromatic cap, respectively. The 24-nt siRNA length dependence relies on the separation between the 5′-phosphorylated end of the guide RNA and dual cleavage sites formed by the paired ribonuclease III domains. These structural studies, complemented by functional data, provide insight into the dicing principle for Dicers in general.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.