Evidence map›Paper›PMID 34647247›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Spore Preparation and Protoplast Isolation to Study Gravity Perception and Response in Ceratopteris richardii.

Ashley E Cannon, Tanya Sabharwal, Stanley J Roux

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Optimization of Isolation and Transformation of Protoplasts fromInternational journal of molecular sciences · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Ashley E Cannon *United States Department of Agriculture, Agriculture Research Service, Wheat Health, Genetics, and Quality Research Unit, Pullman, WA, USA. ashley.cannon@usda.gov.
Tanya Sabharwal *Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.
Stanley J RouxDepartment of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.
The University of Texas at Austin · USQuality Research · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Early studies revealed a highly predictable pattern of gravity-directed growth and development in Ceratopteris richardii spores. This makes the spore a valuable model system for the study of how a single-cell senses and responds to the force of gravity. Gravity regulates both the direction and magnitude of a trans-cell calcium current in germinating spores, and the orientation of this current predicts the polarization of spore development. In order to make Ceratopteris richardii cells easier to transform and image during this developmental process, a procedure for isolating protoplasts from Ceratopteris richardii gametophytes has been developed and optimized. These protoplasts follow the same developmental pattern as Ceratopteris richardii spores and can be used to monitor the molecular and developmental processes during single-cell polarization. Here, we describe this optimized procedure, along with protocols for sterilizing the spores, sowing them in solid or liquid growth media, and evaluating germination and polarization.

Indexed as

Gravity SensingPteridaceaeCell PolarityProtoplastsSporesCalciumCeratopteris richardiiGravity-directed developmentPolarizationProtoplast isolationSingle cell

Identifiers

PMID34647247
OpenAlexW3206745098

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.