ArticleReproductive medicine and biology2021
Effect of docosahexaenoic acid on in vitro growth of bovine oocytes.
Article in Reproductive medicine and biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 7 citations in OpenAlex.
- Effects of Insulin-like Growth Factor I and Follicular Fluid on In Vitro Growth of Cultured Oocytes.Biology · 2025Article
- Role of functional fatty acids in modulation of reproductive potential in livestock.Journal of animal science and biotechnology · 2023Review
- Effect of docosahexaenoic acid on in vitro growth of bovine oocytes.Reproductive medicine and biology · 2021Article
- Current Status of In Vitro Oocyte Growth and Development in Mammals.Reproductive medicine and biologyReview
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe present study investigated the effects of docosahexaenoic acid (DHA) on the growth of bovine oocytes.
methodsOocytes and granulosa cell complexes (OGCs) were collected from early antral follicles (0.4-0.7 mm) on the surface of ovaries harvested from a slaughterhouse. The OGCs were cultured with 0, 1, and 10 μmol/L docosahexanoic acid (DHA) for 16 days.
resultsAntrum formation of the OGCs and the number of granulosa cells (GCs) surrounding the oocytes were comparable among groups, whereas supplementation of 0.1 μmol/L of DHA significantly improved oocyte growth. Oocytes grown with DHA had a higher fertilization rate, acetylation levels of H4K12, and ATP contents, as well as a lower lipid content compared with those grown without DHA. In addition, GCs surrounding OGCs grown with DHA had low lipid content compared with vehicle counterparts. Furthermore, when GCs were cultured in vitro, DHA increased ATP production, mitochondrial membrane potential, and reduced lipid content and levels of reactive oxygen species. RNA-seq of GCs revealed that DHA increased
conclusionThe results suggest that DHA supplementation affects granulosa cell characteristics and supports oocyte growth in vitro.
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Registered trials
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