ArticleScientific reports2021
A novel temporary immersion bioreactor system for large scale multiplication of banana (Rasthali AAB-Silk).
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- Somatic embryogenesis of Arabica coffee in temporary immersion culture: Advances, limitations, and perspectives for mass propagation of selected genotypes.Frontiers in plant science · 2022Pooled it
- Bioreactor Technology for Medicinal Plant In Vitro Cultures: Systems, Applications, and Future Perspectives.Biology · 2026Review
- In Vitro regeneration of bio-immunized banana cv. Grand Naine using a novel double-decker temporary immersion bioreactor.Scientific reports · 2026Article
- Evaluation of Different Bioreactors During In Vitro Shoot Proliferation and Acclimatization of Agave (Plants (Basel, Switzerland) · 2026Article
- Review
- Revealing and characterizing bacterial communities of in vitro Musa species through 16S rDNA metabarcoding and culture dependent approaches.Scientific reports · 2026Article
- Article
- Bioreactor Systems to Mass-Produce the Undervalued Crop,Engineering in life sciences · 2025Article
- Reprogramming Hairy Root Cultures: A Synthetic Biology Framework for Precision Metabolite Biosynthesis.Plants (Basel, Switzerland) · 2025Review
- Temporary immersion systems induce photomixotrophism during in vitro propagation of agave Tobalá.3 Biotech · 2024Article
- Bulbil initiation: a comprehensive review on resources, development, and utilisation, with emphasis on molecular mechanisms, advanced technologies, and future prospects.Frontiers in plant science · 2024Review
- Biochemical and Molecular Characterization ofPlants (Basel, Switzerland) · 2023Article
- Successful In Vitro Shoot Multiplication ofPlants (Basel, Switzerland) · 2023Article
- An Efficient Method ofPlants (Basel, Switzerland) · 2023Article
- Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review.Plants (Basel, Switzerland) · 2023Review
- Bioreactor systems for micropropagation of plants: present scenario and future prospects.Frontiers in plant science · 2023Review
- A Temporary Immersion System to ImproveFrontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Musa sp. cultivar Rasthali (Silk AAB) is a choice variety of the Asian sub-continent. Its production and sustenance are threatened by Fusarium wilt, which affects the livelihoods of small and marginal farmers. The use of quality planting material is one of the strategies to manage the disease. Availability of quality planting material for varieties other than Grand Naine is limited. Large-scale micropropagation using existing technologies is laborious and expensive. Temporary immersion bioreactor system is emerging as a potential advancement in the micropropagation industry. In this study, a cost-effective temporary immersion bioreactor (TIB) system has been developed and an efficient micropropagation method has been standardized. Explants cultured in TIB with 250 ml of culture medium in a 2-min immersion frequency of 6 h were found to be efficient for shoot proliferation and rooting. Its efficacy has been compared with the semisolid culture method. At the end of the 6th subculture, 1496 ± 110 shoots per explant were obtained in TIB. Chlorophyll, carotenoid, stomatal index, and the number of closed stomata were examined to determine the physiological functions of the plants grown in TIB and compared with semisolid grown plantlets. Plantlets grown in TIB were genetically stable and were confirmed using inter-simple sequence repeat (ISSR) markers. The multiplication of shoots in TIB was 2.7-fold higher than the semisolid culture method, which is suitable for large-scale production of planting material for commercial applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.