Evidence map›Paper›PMID 34645492›Full record

ArticleParasites & vectors2021

Aedes aegypti: egg morphology and embryonic development.

Ana Paula Miranda Mundim-Pombo, Hianka Jasmyne Costa de Carvalho, Rafaela Rodrigues Ribeiro, Marisol León, Durvanei Augusto Maria, Maria Angélica Miglino

Open access · goldAbstract read
In one paragraph

Article in Parasites & vectors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
4.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 27 citations in OpenAlex.

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  9. Targeting the vector of arbovirusesBeilstein journal of nanotechnology · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Ana Paula Miranda Mundim-PomboUniversity Nilton Lins, Manaus, Amazonas, Brazil.
Hianka Jasmyne Costa de CarvalhoDepartment of Surgery, Anatomy Sector, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, Avenida Professor Orlando Marques de Paiva, 87, Butantã, São Paulo, São Paulo, CEP 05508-270, Brazil.
Rafaela Rodrigues RibeiroDepartment of Surgery, Anatomy Sector, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, Avenida Professor Orlando Marques de Paiva, 87, Butantã, São Paulo, São Paulo, CEP 05508-270, Brazil.
Marisol LeónDepartment of Surgery, Anatomy Sector, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, Avenida Professor Orlando Marques de Paiva, 87, Butantã, São Paulo, São Paulo, CEP 05508-270, Brazil.
Durvanei Augusto MariaMolecular Biology Laboratory of the Butantan Institute, São Paulo, Brazil.
Maria Angélica MiglinoDepartment of Surgery, Anatomy Sector, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, Avenida Professor Orlando Marques de Paiva, 87, Butantã, São Paulo, São Paulo, CEP 05508-270, Brazil. miglino@usp.br.ORCID http://orcid.org/0000-0003-4979-115X
Universidade de São Paulo · BRInstituto Butantan · BRUniversidade Nilton Lins · BR

Funding

Fundação de Amparo à Pesquisa do Estado do Amazonas 147941081115022013
6 · The paper itself

Abstract

backgroundThe diseases for which Aedes aegypti is a vector are worrisome. The high vector competence of this mosquito, as well as its anthropophilia and ability to adapt to the urban environment, allows it to exploit many habitats, making its prevention an arduous task. Despite current disease control measures focused on the mosquito, the effectiveness in containing its dispersion still requires improvement; thus greater knowledge about this insect is fundamental.

methodsAedes aegypti egg morphology and embryonic development were analyzed from eggs of the insectary of the Institute of Biomedical Sciences of the University of São Paulo. Optical (light and confocal) and electronic (transmission and scanning) microscopy were used to analyze the morphological and ultrastructural features of the eggs. Embryos were observed in the initial (0-20.5 h after egg-laying), intermediate (20.6-40.1 h after egg-laying), and final (40.2-61.6 h) stages of development, and kept at a temperature of 28 °C ± 1 °C until collection for processing.

resultsEggs of Ae. aegypti were whitish at the time of oviposition, and then quickly became black. The egg length was 581.45 ± 39.73 μm and the width was 175.36 ± 11.59. Access to the embryo was difficult due to the egg morphology, point of embryonic development, and difficult permeability of the exochorion (mainly in fixation). Only about 5% of the collected eggs were successfully processed. In the initial stage of embryonic development, characteristics suggestive of intense cellular activity were found. In the intermediate stage, the beginning of the segmentation process was evident. In the final phase, it was possible to differentiate the cephalic region and the thoracic and abdominal segments.

conclusionThe chorion was found to be an important protective barrier and a limiting factor for the evaluation of the embryos and mosquito embryonic cells, indicating that further studies need to be carried out to identify the reason that this occurs.

Indexed as

Embryonic DevelopmentAedesAnimalsDengueFemaleMosquito VectorsOvipositionOvumArbovirusesEggs of Aedes aegyptiEntomology ultrastructural analysisPublic health

Identifiers

PMID34645492
PMCPMC8515647
OpenAlexW3207269782

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.