Evidence map›Paper›PMID 34643932›Full record

ArticleMolecular biology reports2021

Next-generation sequencing, isolation and characterization of 14 microsatellite loci of Canthon cyanellus (Coleoptera: Scarabaeidae).

Luis Rodrigo Arce-Valdés, Rosa Ana Sánchez-Guillén, Janet Nolasco-Soto, Mario E Favila

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Luis Rodrigo Arce-ValdésInstituto de Ecología, A.C. Red de Biología Evolutiva, Xalapa, Veracruz, Mexico.ORCID http://orcid.org/0000-0001-6445-7534
Rosa Ana Sánchez-GuillénInstituto de Ecología, A.C. Red de Biología Evolutiva, Xalapa, Veracruz, Mexico.ORCID http://orcid.org/0000-0001-6024-8321
Janet Nolasco-SotoInstituto de Ecología, A.C. Red de Biología Evolutiva, Xalapa, Veracruz, Mexico.ORCID http://orcid.org/0000-0002-5979-6598
Mario E FavilaInstituto de Ecología, A.C. Red de Ecoetología, Xalapa, Veracruz, Mexico. mario.favila@inecol.mx.ORCID http://orcid.org/0000-0002-2737-9327

Funding

Consejo Nacional de Ciencia y Tecnología CB 282922
6 · The paper itself

Abstract

backgroundWe used Illumina paired-end sequencing to isolate and characterize microsatellites of Canthon cyanellus, a Neotropical roller dung beetle, encompassing several lineages within its distribution range. METHODS AND

resultsWe examined C. cyanellus specimens collected at eight different localities in Mexico (two or three specimens per locality). We initially performed amplification tests with 16 loci, but two of which were unsuccessful. The 14 remaining microsatellites were polymorphic, with 2-16 alleles each. The expected and observed heterozygosity ranged from 0.11 to 0.76 and from 0.20 to 0.78, respectively.

conclusionsThese microsatellites will help to assess structure at the population and lineage levels, identify zones of potential hybridization between lineages, and draw a more precise geographic delimitation of C. cyanellus lineages.

Indexed as

Microsatellite RepeatsAllelesAnimalsColeopteraGenetic LociHeterozygoteHigh-Throughput Nucleotide SequencingSequence Analysis, DNACanthon cyanellusIsolationMexicoMicrosatellites

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.