Evidence map›Paper›PMID 34643926›Full record

ArticleMolecular biology reports2021

Isolation and characterization of microsatellites markers in Centaurium grandiflorum ssp. boissieri.

Vania Jiménez-Lobato, Marcial Escudero, Zoila Díaz Lifante, Cristina Andrés Camacho, Alejandra de Castro, Juan Arroyo

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Article in Molecular biology reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Vania Jiménez-LobatoLaboratorio Nacional de Análisis y Síntesis Ecológica - Campus Costa Grande, Escuela Superior de Desarrollo Sustentable, Universidad Autónoma de Guerrero, Cátedras - CONACYT, Carretera Federal Acapulco - Zihuatanejo, Las Tunas, Tecpan de Galeana, Guerrero, México. vaniajimenez@uagro.mx.ORCID http://orcid.org/0000-0003-2050-9026
Marcial EscuderoDepartamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain.
Zoila Díaz LifanteDepartamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain.
Cristina Andrés CamachoDepartamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain.
Alejandra de CastroDepartamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain.
Juan ArroyoDepartamento de Biología Vegetal y Ecología, Facultad de Biología, Universidad de Sevilla, Sevilla, Spain.

Funding

Agencia Estatal de Investigación CGL2013-4503-PAgencia Estatal de Investigación PGC2018-099608-B-100
6 · The paper itself

Abstract

backgroundEstimating outcrossing/selfing rates and characterizing genetic diversity with microsatellite markers are crucial to understanding the evolution of plant mating systems. METHODS AND

resultsWe developed, optimized and characterized eight new primer pairs for Centaurium grandiflorum ssp. boissieri and transferred them to three subspecies of Centaurium quadrifolium. Two SSR loci were transferred from Sabatia campestris to the four Centaurium taxa. Polymorphisms, H

conclusionsThis is the first report of microsatellites successfully amplified in the whole Centaurium genus. They will be valuable for estimating mating system parameters and genetic diversity and exploring their relationships with the wide variation in flower morphology in the genus, especially anther-stigma separation.

Indexed as

AllelesCentauriumFlowersGenetic LociGenetic VariationHybridization, GeneticMicrosatellite RepeatsPolymorphism, GeneticCentauriumGentianaceaeMating systemsSSR markers

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