ArticleChembiochem : a European journal of chemical biology2022
Defluorination Capability of l-2-Haloacid Dehalogenases in the HAD-Like Hydrolase Superfamily Correlates with Active Site Compactness.
Article in Chembiochem : a European journal of chemical biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Decoding cryptic defluorinases through a latent generative sequence landscape.Chemical science · 2026Article
- Current Research Advances and Future Prospects on Microbial Consortia for Sustainable PFAS Remediation.International journal of molecular sciences · 2026Review
- Transaminase-catalyzed sequential hydrodefluorinations of perfluorinated methylene groups.Communications chemistry · 2025Article
- Microbially-mediated halogenation and dehalogenation cycling of organohalides in the ocean.Nature communications · 2025Article
- Continuous Spectrophotometric Assay for Defluorinase and Dechlorinase Activities With α-Halocarboxylic Acids.Microbial biotechnology · 2025Article
- Enzymatic carbon-fluorine bond cleavage by human gut microbes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Computational Studies of Enzymes for C-F Bond Degradation and Functionalization.Chemphyschem : a European journal of chemical physics and physical chemistry · 2025Review
- Metagenome-Assembled Genomes and Metatranscriptome Analysis of Perfluorooctane Sulfonate-Reducing Bacteria Enriched From Activated Sludge.Environmental microbiology · 2025Article
- Article
- A prescription for engineering PFAS biodegradation.The Biochemical journal · 2024Review
- Phenotypic Plasticity During Organofluorine Degradation Revealed by Adaptive Evolution.Microbial biotechnology · 2024Article
- The link between ancient microbial fluoride resistance mechanisms and bioengineering organofluorine degradation or synthesis.Nature communications · 2024Review
- Toward the development of a molecular toolkit for the microbial remediation of per-and polyfluoroalkyl substances.Applied and environmental microbiology · 2024Review
- Evolutionary obstacles and not C-F bond strength make PFAS persistent.Microbial biotechnology · 2024Article
- Reanalysis of a μ opioid receptor crystal structure reveals a covalent adduct with BU72.BMC biology · 2023Article
- X-ray structure and mechanism of ZgHAD, a l-2-haloacid dehalogenase from the marine Flavobacterium Zobellia galactanivorans.Protein science : a publication of the Protein Society · 2023Article
- Chemical Diversity and Biosynthesis of Drimane-Type Sesquiterpenes in the Fungal Kingdom.Chembiochem : a European journal of chemical biology · 2022Review
- Strategies for the Biodegradation of Polyfluorinated Compounds.Microorganisms · 2022Review
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Authors and funding
13 authors at 8 institutions in 3 countries.
Funding
Abstract
l-2-Haloacid dehalogenases, industrially and environmentally important enzymes that catalyse cleavage of the carbon-halogen bond in S-2-halocarboxylic acids, were known to hydrolyse chlorinated, brominated and iodinated substrates but no activity towards fluorinated compounds had been reported. A screen for novel dehalogenase activities revealed four l-2-haloacid dehalogenases capable of defluorination. We now report crystal structures for two of these enzymes, Bpro0530 and Rha0230, as well as for the related proteins PA0810 and RSc1362, which hydrolyse chloroacetate but not fluoroacetate, all at ∼2.2 Å resolution. Overall structure and active sites of these enzymes are highly similar. In molecular dynamics (MD) calculations, only the defluorinating enzymes sample more compact conformations, which in turn allow more effective interactions with the small fluorine atom. Structural constraints, based on X-ray structures and MD calculations, correctly predict the defluorination activity of the homologous enzyme ST2570.
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Registered trials
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