Observational studyJournal of assisted reproduction and genetics2021
Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study.
Observational study in Journal of assisted reproduction and genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Oocyte activation for women following intracytoplasmic sperm injection (ICSI).The Cochrane database of systematic reviews · 2024Pooled it
- Article
- Add-On Technologies That Aim to Improve Oocyte Quality and Embryo Implantation Potential.Medicina (Kaunas, Lithuania) · 2025Review
- Good practice recommendations on add-ons in reproductive medicine†.Human reproduction (Oxford, England) · 2023Article
- Significant differences in efficiency between two commonly used ionophore solutions for assisted oocyte activation (AOA): a prospective comparison of ionomycin and A23187.Journal of assisted reproduction and genetics · 2023Article
- Early rescue oocyte activation at 5 h post-ICSI is a useful strategy for avoiding unexpected fertilization failure and low fertilization in ICSI cycles.Frontiers in endocrinology · 2023Observational
- Artificial oocyte activation with CaFrontiers in endocrinology · 2023Article
- Effect of A23187 ionophore treatment on human blastocyst development-a sibling oocyte study.Journal of assisted reproduction and genetics · 2022Article
- Oocyte activation deficiency and assisted oocyte activation: mechanisms, obstacles and prospects for clinical application.Human reproduction open · 2022Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo evaluate whether ionophore application at the oocyte stage changes the morphokinetics of the associated embryos in cases of artificial oocyte activation.
methodsIn a prospective sibling oocyte approach, 78 ICSI patients with suspected fertilization problems had half of their MII-oocytes treated with a ready-to-use ionophore (calcimycin) immediately following ICSI (study group). Untreated ICSI eggs served as the control group. Primary analyses focused on morphokinetic behavior and the presence of irregular cleavages. The rates of fertilization, utilization, pregnancy, and live birth rate were also evaluated.
resultsIonophore-treated oocytes showed a significantly earlier formation of pronuclei (t2PNa) and a better synchronized third cell cycle (s3) (P < .05). The rate of irregular cleavage was unaffected (P > .05). Ionophore treatment significantly improved the overall rates of fertilization (P < .01) and blastocyst utilization (P < .05).
conclusionIonophore application does not negatively affect cleavage timing nor is it associated with irregular cleavage.
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Registered trials
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