ArticleInternational journal of molecular sciences2021
Tissue Sampling and Homogenization in the Sub-Microliter Scale with a Nanosecond Infrared Laser (NIRL) for Mass Spectrometric Proteomics.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Infrared laser sampling of low volumes combined with shotgun lipidomics reveals lipid markers in palatine tonsil carcinoma.Molecular oncology · 2026Article
- Nanosecond Infrared Laser Sampling of Mouse and Human Liver Tissues for LC-MS/MS Analysis of Bile Acids.International journal of molecular sciences · 2026Article
- Layer-Specific Proteomic Profiling of the Human Cornea Reveals Insights Into Structure and Biological Function.Investigative ophthalmology & visual science · 2026Article
- Clinical glycoproteomics: methods and diseases.MedComm · 2024Review
- Optimization of lung tissue pre-treatment by bead homogenization for subsequent culturomics.Scientific reports · 2024Article
- Nanosecond infrared laser (NIRL) for cutting roots of human teeth: thermal effects and quality of cutting edges.Lasers in medical science · 2024Article
- Direct 3D Sampling of the Embryonic Mouse Head: Layer-wise Nanosecond Infrared Laser (NIRL) Ablation from Scalp to Cortex for Spatially Resolved Proteomics.Analytical chemistry · 2023Article
- Lipidome Analysis of Oropharyngeal Tumor Tissues Using Nanosecond Infrared Laser (NIRL) Tissue Sampling and Subsequent Mass Spectrometry.International journal of molecular sciences · 2023Article
- Article
- Tissue Sampling and Homogenization with NIRL Enables Spatially Resolved Cell Layer Specific Proteomic Analysis of the Murine Intestine.International journal of molecular sciences · 2022Article
- Infrared Laser Ablation Microsampling with a Reflective Objective.Journal of the American Society for Mass Spectrometry · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
It was recently shown that ultrashort pulse infrared (IR) lasers, operating at the wavelength of the OH vibration stretching band of water, are highly efficient for sampling and homogenizing biological tissue. In this study we utilized a tunable nanosecond infrared laser (NIRL) for tissue sampling and homogenization with subsequent liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis for mass spectrometric proteomics. For the first time, laser sampling was performed with murine spleen and colon tissue. An ablation volume of 1.1 × 1.1 × 0.4 mm³ (approximately 0.5 µL) was determined with optical coherence tomography (OCT). The results of bottom-up proteomics revealed proteins with significant abundance differences for both tissue types, which are in accordance with the corresponding data of the Human Protein Atlas. The results demonstrate that tissue sampling and homogenization of small tissue volumes less than 1 µL for subsequent mass spectrometric proteomics is feasible with a NIRL.
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