ArticleInternational journal of molecular sciences2021
Single-Tear Proteomics: A Feasible Approach to Precision Medicine.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Tear Fluid as a Matrix for Biomonitoring Environmental and Chemical Exposures.Current environmental health reports · 2024Pooled it
- Canine Tear Proteomics: A New Frontier in Veterinary Ophthalmology.Veterinary ophthalmology · 2026Review
- Exploratory multi-biofluid proteomic profiling of acute exercise responses in plasma, tear fluid, and nasal secretions.Frontiers in physiology · 2026Article
- Precision in Tear Fluid Biomarker Discovery: Quantitative Proteomic Profiling of Small-Volume, Individual Samples Using Capillary Tube Collection.Biomedicines · 2025Article
- Ocular surface microbiota alterations in patients with pterygium.Frontiers in cellular and infection microbiology · 2025Article
- Development, Optimization, and Clinical Relevance of Lactoferrin Delivery Systems: A Focus on Ocular Delivery.Pharmaceutics · 2024Review
- Sources of Variance in Human Tear Proteomic Samples: Statistical Evaluation, Quality Control, Normalization, and Biological Insight.International journal of molecular sciences · 2024Article
- NanoLC-timsTOF-Assisted Analysis of Glycated Albumin in Diabetes-Affected Plasma and Tears.Journal of the American Society for Mass Spectrometry · 2024Article
- The Potential Utility of Salivary and Tear Proteomics to Discriminate Sjögren's Disease from Non-Sjögren's Sicca.International journal of molecular sciences · 2023Review
- Profiling tear film enzymes reveals major metabolic pathways involved in the homeostasis of the ocular surface.Scientific reports · 2023Article
- Targeted Workflow Investigating Variations in the Tear Proteome by Liquid Chromatography Tandem Mass Spectrometry.ACS omega · 2023Article
- Evaluation of pre-processing methods for tear fluid proteomics using proximity extension assays.Scientific reports · 2023Article
- Advances in clinical examination of lacrimal gland.Frontiers in medicine · 2023Review
- Mass Spectrometric Proteomics 2022.International journal of molecular sciences · 2022Article
- Contact Lens Wear Induces Alterations of Lactoferrin Functionality in Human Tears.Pharmaceutics · 2022Article
- Metabolic phenotyping of tear fluid as a prognostic tool for personalised medicine exemplified by T2DM patients.The EPMA journal · 2022Article
- A Review of Emerging Tear Proteomics Research on the Ocular Surface in Ocular Allergy.Biology · 2022Review
- Tear-Based Vibrational Spectroscopy Applied to Amyotrophic Lateral Sclerosis.Analytical chemistry · 2021Article
- Proteomic Analysis of Tears and Conjunctival Cells Collected with Schirmer Strips Using timsTOF Pro: Preanalytical Considerations.Metabolites · 2021Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lacrimal fluid is an attractive source of noninvasive biomarkers, the main limitation being the small sample amounts typically collected. Advanced analytical methods to allow for proteomics profiling from a few microliters are needed to develop innovative biomarkers, with attractive perspectives of applications to precision medicine. This work describes an effective, analytical pipeline for single-tear analysis by ultrahigh-resolution, shotgun proteomics from 23 healthy human volunteers, leading to high-confidence identification of a total of 890 proteins. Highly reproducible quantification was achieved by either peak intensity, peak area, or spectral counting. Hierarchical clustering revealed a stratification of females vs. males that did not emerge from previous studies on pooled samples. Two subjects were monitored weekly over 3 weeks. The samples clustered by withdrawal time of day (morning vs. afternoon) but not by follow-up week, with elevated levels of components of the immune system in the morning samples. This study demonstrates feasibility of single-tear quantitative proteomics, envisaging contributions of this unconventional body fluid to individualized approaches in biomedicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.