Evidence map›Paper›PMID 34638562›Full record

ArticleInternational journal of molecular sciences2021

Comparative Analysis of Genome Editors Efficiency on a Model of Mice Zygotes Microinjection.

Olga A Averina, Oleg A Permyakov, Olga O Grigorieva, Alexey S Starshin, Alexander M Mazur, Egor B Prokhortchouk, Olga A Dontsova, Petr V Sergiev

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. CRISPR/Cas9 Delivery Systems to Enhance Gene Editing Efficiency.International journal of molecular sciences · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Olga A AverinaInstitute of Functional Genomics, Lomonosov Moscow State University, 119991 Moscow, Russia.
Oleg A PermyakovInstitute of Functional Genomics, Lomonosov Moscow State University, 119991 Moscow, Russia.
Olga O GrigorievaInstitute of Functional Genomics, Lomonosov Moscow State University, 119991 Moscow, Russia.
Alexey S StarshinResearch Center of Biotechnology of the Russian Academy of Sciences, Institute of Bioengineering, 119071 Moscow, Russia.ORCID 0000-0002-4965-0165
Alexander M MazurResearch Center of Biotechnology of the Russian Academy of Sciences, Institute of Bioengineering, 119071 Moscow, Russia.
Egor B ProkhortchoukResearch Center of Biotechnology of the Russian Academy of Sciences, Institute of Bioengineering, 119071 Moscow, Russia.
Olga A DontsovaDepartment of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia.
Petr V SergievInstitute of Functional Genomics, Lomonosov Moscow State University, 119991 Moscow, Russia.ORCID 0000-0001-8866-1863

Funding

Russian Science Foundation 21-64-00006
6 · The paper itself

Abstract

Genome editing is an indispensable tool for functional genomics. The caveat of the genome-editing pipeline is a prevalence of error-prone non-homologous end joining over homologous recombination, while only the latter is suitable to introduce particularly desired genetic variants. To overcome this problem, a toolbox of genome engineering was appended by a variety of improved instruments. In this work, we compared the efficiency of a number of recently suggested improved systems for genome editing applied to the same genome regions on a murine zygote model via microinjection. As a result, we observed that homologous recombination utilizing an ssDNA template following sgRNA directed Cas9 cleavage is still the method of choice for the creation of animals with precise genome alterations.

Indexed as

AnimalsCRISPR-Cas SystemsDNA End-Joining RepairDNA, Single-StrandedGene EditingHomologous RecombinationMiceMicroinjectionsModels, AnimalRNA, Guide, CRISPR-Cas SystemsZygoteDNA, Single-StrandedRNA, Guide, CRISPR-Cas SystemsCRISPR Cas9genome editinghomologous recombinationknockout micemicroinjectionprime editor

Identifiers

PMID34638562
PMCPMC8508852

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.