ArticleGenomics & informatics2021
Rapid and sensitive detection of Salmonella species targeting the hilA gene using a loop-mediated isothermal amplification assay.
Article in Genomics & informatics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Genogroup-Specific Multiplex Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay for Point-of-Care Detection of Norovirus.Diagnostics (Basel, Switzerland) · 2025Article
- A Colorimetric LAMP Assay forFoods (Basel, Switzerland) · 2025Article
- Research progress of loop-mediated isothermal amplification in the detection of Salmonella for food safety applications.Discover nano · 2024Review
- Establishment of a Simple, Sensitive, and SpecificFoods (Basel, Switzerland) · 2024Article
- A One-Pot Convenient RPA-CRISPR-Based Assay forMicroorganisms · 2024Article
- Comparison of loop-mediated isothermal amplification, multiplex PCR, and REP- PCR techniques for identification of carbapenem-resistantIranian journal of microbiology · 2023Article
- Development of reverse-transcription loop-mediated isothermal amplification assays for point-of-care testing of human influenza virus subtypes H1N1 and H3N2.Genomics & informatics · 2022Article
- Rapid and specific detection ofMethodsX · 2022Article
- Qualitative and Quantitative Detection of Potentially VirulentPathogens (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Salmonella species are among the major pathogens that cause foodborne illness outbreaks. In this study, we aimed to develop a loop-mediated isothermal amplification (LAMP) assay for the rapid and sensitive detection of Salmonella species. We designed LAMP primers targeting the hilA gene as a universal marker of Salmonella species. A total of seven Salmonella species strains and 11 non-Salmonella pathogen strains from eight different genera were used in this study. All Salmonella strains showed positive amplification signals with the Salmonella LAMP assay; however, there was no non-specific amplification signal for the non-Salmonella strains. The detection limit was 100 femtograms (20 copies per reaction), which was ~1,000 times more sensitive than the detection limits of the conventional polymerase chain reaction (PCR) assay (100 pg). The reaction time for a positive amplification signal was less than 20 minutes, which was less than one-third the time taken while using conventional PCR. In conclusion, our Salmonella LAMP assay accurately detected Salmonella species with a higher degree of sensitivity and greater rapidity than the conventional PCR assay, and it may be suitable for point-of-care testing in the field.
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