Evidence map›Paper›PMID 34637792›Full record

ArticleLife sciences2021

UVB-induced eIF2α phosphorylation in keratinocytes depends on decreased ATF4, GADD34 and CReP expression levels.

Verónica A Bahamondes Lorca, Bernardo D Bastidas Mayorga, Lingying Tong, Shiyong Wu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Life sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Verónica A Bahamondes LorcaEdison Biotechnology Institute, Ohio University, Athens, OH 45701, USA; Department of Chemistry and Biochemistry, Ohio University, Athens, OH 45701, USA; Departamento de Tecnología Médica, Facultad de Medicina, Universidad de Chile, Santiago, Chile.
Bernardo D Bastidas MayorgaEdison Biotechnology Institute, Ohio University, Athens, OH 45701, USA; Department of Chemistry and Biochemistry, Ohio University, Athens, OH 45701, USA.
Lingying TongEdison Biotechnology Institute, Ohio University, Athens, OH 45701, USA; Department of Chemistry and Biochemistry, Ohio University, Athens, OH 45701, USA. Electronic address: tongl1@ohio.edu.
Shiyong WuEdison Biotechnology Institute, Ohio University, Athens, OH 45701, USA; Department of Chemistry and Biochemistry, Ohio University, Athens, OH 45701, USA. Electronic address: wus1@ohio.edu.
Biotechnology Institute · USOhio University · US

Funding

UV INDUCED AND PERK MEDIATED SIGNALING PATHWAYSR01CA086928 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WU, SHIYONG · 2000 to 2012
$2.1M
Molecular mechanisms of cNOS-mediated NF-kappa B activation in regulation of ultraviolet B light-induced photocarcinogenic responsesR01ES030425 · NIEHS · OHIO UNIVERSITY ATHENS · PI WU, SHIYONG · 2019 to 2023
$1.7M
UV-induced and NOS-mediated Zn elevation, translation regulatin and apoptosis.R56CA086928 · NCI · OHIO UNIVERSITY ATHENS · PI WU, SHIYONG · 2007 to 2007
$206k
NCI NIH HHS R01 CA086928NCI NIH HHS R56 CA086928NIEHS NIH HHS R01 ES030425
6 · The paper itself

Abstract

aimTo elucidate the mechanism behind the sustained high levels of phosphorylated eIF2α in HaCaT cells post-UVB. MAIN

methodsIn this study, expression levels of the machinery involved in the dephosphorylation of eIF2α (GADD34, CReP and PP1), as well as the PERK-eIF2α-ATF4-CHOP, IRE1α/XBP1s and ATF6α signaling cascades, were analyzed by western blot and fluorescence microscope. KEY

findingsOur data showed that UVB induces the phosphorylation of eIF2α, which induces the translation of ATF4 and consequently the expression of CHOP and GADD34. Nevertheless, UVB also suppresses the translation of ATF4 and GADD34 in HaCaT cells via a p-eIF2α independent mechanism. Therefore, the lack of ATF4, GADD34 and CReP is responsible for the sustained phosphorylation of eIF2α. Finally, our data also showed that UVB selectively modifies PERK and downregulates ATF6α expression but does not induce activation of the IRE1α/XBP1s pathway in HaCaT cells. SIGNIFICANCE: Novel mechanism to explain the prolonged phosphorylation of eIF2α post-UVB irradiation.

Indexed as

Activating Transcription Factor 4Cell LineeIF-2 KinaseEndoribonucleasesEukaryotic Initiation Factor-2Gene ExpressionGene Expression RegulationHumansKeratinocytesPhosphorylationProtein BiosynthesisProtein Phosphatase 1Protein Serine-Threonine KinasesSignal TransductionTranscription Factor CHOPUltraviolet RaysActivating Transcription Factor 4ATF4 protein, humaneIF-2 KinaseEndoribonucleasesEukaryotic Initiation Factor-2PPP1R15A protein, humanProtein Phosphatase 1Protein Serine-Threonine KinasesTranscription Factor CHOPATF4eIF2αGADD34KeratinocytesUltraviolet radiation B

Identifiers

PMID34637792
PMCPMC8969208
OpenAlexW3207607635

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.