Evidence map›Paper›PMID 34637168›Full record

ArticleChembiochem : a European journal of chemical biology2022

Cell-Free Multi-Enzyme Synthesis and Purification of Uridine Diphosphate Galactose.

Reza Mahour, Ju Weon Lee, Pia Grimpe, Simon Boecker, Valerian Grote, Steffen Klamt, Andreas Seidel-Morgenstern, Thomas F T Rexer, Udo Reichl

Abstract read
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Multienzyme Platform for the Synthesis of UDP Sugars and Human Milk Oligosaccharides.Chembiochem : a European journal of chemical biology · 2026
    Article
  2. Cell-Free Multi-Enzyme Synthesis and Purification of Uridine Diphosphate Galactose.Chembiochem : a European journal of chemical biology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Reza MahourMax Planck Institute for Dynamics of Complex Technical Systems, Department of Bioprocess Engineering, Sandtorstrasse 1, 39106, Magdeburg, Germany.
Ju Weon LeeMax Planck Institute for Dynamics of Complex Technical Systems, Department of Physical and Chemical Foundations of Process Engineering, Sandtorstrasse 1, 39106, Magdeburg, Germany.
Pia GrimpeMax Planck Institute for Dynamics of Complex Technical Systems, Department of Bioprocess Engineering, Sandtorstrasse 1, 39106, Magdeburg, Germany.
Simon BoeckerMax Planck Institute for Dynamics of Complex Technical Systems, Research group Analysis and Redesign of Biological Networks, Sandtorstrasse 1, 39106, Magdeburg, Germany.
Valerian GroteMax Planck Institute for Dynamics of Complex Technical Systems, Department of Bioprocess Engineering, Sandtorstrasse 1, 39106, Magdeburg, Germany.
Steffen KlamtMax Planck Institute for Dynamics of Complex Technical Systems, Research group Analysis and Redesign of Biological Networks, Sandtorstrasse 1, 39106, Magdeburg, Germany.
Andreas Seidel-MorgensternMax Planck Institute for Dynamics of Complex Technical Systems, Department of Physical and Chemical Foundations of Process Engineering, Sandtorstrasse 1, 39106, Magdeburg, Germany.
Thomas F T RexerMax Planck Institute for Dynamics of Complex Technical Systems, Department of Bioprocess Engineering, Sandtorstrasse 1, 39106, Magdeburg, Germany.ORCID 0000-0002-2105-1283
Udo ReichlMax Planck Institute for Dynamics of Complex Technical Systems, Department of Bioprocess Engineering, Sandtorstrasse 1, 39106, Magdeburg, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

High costs and low availability of UDP-galactose hampers the enzymatic synthesis of valuable oligosaccharides such as human milk oligosaccharides. Here, we report the development of a platform for the scalable, biocatalytic synthesis and purification of UDP-galactose. UDP-galactose was produced with a titer of 48 mM (27.2 g/L) in a small-scale batch process (200 μL) within 24 h using 0.02 g

Indexed as

Adenosine TriphosphateBioreactorsCell-Free SystemEnzymesHydrogen-Ion ConcentrationOligosaccharidesProof of Concept StudyUridine Diphosphate GalactoseAdenosine TriphosphateEnzymesOligosaccharidesUridine Diphosphate GalactoseATP regenerationcell-free synthesismulti-enzymes biosynthesissugar nucleotidesUDP-galactose

Identifiers

PMID34637168
PMCPMC9299652

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.