Evidence map›Paper›PMID 34637033›Full record

ArticleInflammation2021

The miR-223-3p Regulates Pyroptosis Through NLRP3-Caspase 1-GSDMD Signal Axis in Periodontitis.

Yiru Xia, Kecong Zhou, Mengjun Sun, Rong Shu, Jielei Qian, Yufeng Xie

Abstract read
PubMed Publisher
In one paragraph

Article in Inflammation, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
2.2field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 46 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Inflammasomes in Oral Pathologies: From Periodontal Disease to Oral Cancer.Advances in experimental medicine and biology · 2026
    Review
  6. Article
  7. Review
  8. Review
  9. Review
  10. Article
  11. Review
  12. Review
  13. MicroRNAs modulation by isodrimeninol fromFrontiers in oral health · 2025
    Article
  14. Review
  15. Article
  16. Review
  17. Review
  18. Review
  19. Emerging roles of exosomes in oral diseases progression.International journal of oral science · 2024
    Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 2 countries.

Yiru Xia *Department of Periodontology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Kecong Zhou *Department of Periodontology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Mengjun SunDepartment of Periodontology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Rong ShuDepartment of Periodontology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Jielei QianDepartment of Periodontology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. kythina@163.com.
Yufeng XieDepartment of Periodontology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. yufengxie_123@163.com.ORCID http://orcid.org/0000-0002-9458-7003
Shanghai Jiao Tong University · CN

Funding

Cross-disciplinary Research Fund of Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine JYJC201904Innovative Research Team on High-level Local Universities in Shanghai SSMU-ZDCX20180900National Natural Science Foundation of China 81991500National Natural Science Foundation of China 81991503Science and Technology Commission of Shanghai Municipality 18ZR1422400
6 · The paper itself

Abstract

Salivary exosomes contain various components and may play important roles in oral diseases. The purpose of this study was to verify the possible function of miR-223-3p from salivary exosomes in periodontitis. We isolated the salivary exosomes and found that the miR-223-3p content of salivary exosomes from periodontitis was less than the healthy control. Furthermore, we performed dual-luciferase reporter assay and real-time PCR to verify that (NOD)-like receptor (NLR) pyrin domain-containing 3 (NLRP3) was the target of miR-223-3p. When we knocked down the miR-223-3p expression in THP-1-derived macrophages, the expression of NLRP3 and the downstream inflammatory mediators interleukin-1β (IL-1β) and IL-6 were upregulated. By using integrated bioinformatics analysis, we found that pyroptosis and cytokine secretion participated in inflammatory gingival tissues. In addition, NLRP3, and the pyroptosis executioner, gasdermin D (GSDMD) was highly active in inflammatory gingival tissues compared with healthy controls by western blotting and immunohistochemistry. In summary, we speculated that miR-223-3p in salivary exosomes might regulate GSDMD-mediated pyroptosis by targeting NLRP3 in periodontitis. Detection of miR-223-3p expression in salivary exosomes could be used as an important non-invasive method to diagnose and evaluate the severity of periodontitis.

Indexed as

PyroptosisCase-Control StudiesCaspase 1ExosomesGasderminsHumansInflammasomesInterleukin-1betaInterleukin-6MacrophagesMicroRNAsNLR Family, Pyrin Domain-Containing 3 ProteinPeriodontitisPhosphate-Binding ProteinsPore Forming Cytotoxic ProteinsSalivaCaspase 1GasderminsGSDMD protein, humanIL1B protein, humanIL6 protein, humanInflammasomesInterleukin-1betaInterleukin-6MicroRNAsMIR223, humanNLR Family, Pyrin Domain-Containing 3 ProteinNLRP3 protein, humanPhosphate-Binding ProteinsPore Forming Cytotoxic ProteinsInflammasomeMicro-RNAPeriodontitisPyroptosisSalivary exosomes

Identifiers

PMID34637033
OpenAlexW3206183968

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.