ArticleNature communications2021
Simultaneous orientation and 3D localization microscopy with a Vortex point spread function.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Optimal excitation polarization schemes to boost the precision of single-molecule orientation imaging.Biomedical optics express · 2026Article
- Single Fluorogens and Orientation-Localization Microscopy for Quantifying Chemical and Biomolecular Dynamics at the Nanoscale.Accounts of chemical research · 2026Article
- Efficient spatio-angular reconstruction enables high-fidelity mapping of six-dimensional structures and dynamics with polarized fluorescence microscopy.Nature communications · 2026Article
- Fused deep learning enables 6D single-molecule localization in polarization-resolved microscopy.Methods and applications in fluorescence · 2026Article
- Chromatix: a differentiable, GPU-accelerated wave-optics library.Nature methods · 2026Article
- Emission dipole orientation reveals dynamic single-molecule interactions with 2D crystals at solvent interfaces.Nature communications · 2026Article
- Review of Machine Learning for Single-Particle Tracking: Methods, Challenges, and Biophysical Insights.Chemical & biomedical imaging · 2026Review
- Chromatix: a differentiable, GPU-accelerated wave-optics library.bioRxiv : the preprint server for biology · 2026Article
- 4polar3D single molecule imaging of 3D orientation in dense actin networks using ratiometric polarization splitting.Nature communications · 2026Article
- Mapping Optical Chirality with Single Fluorescent Molecules.Nano letters · 2026Article
- Separation estimation of two freely rotating dipole emitters near the quantum limit.Physical review. A · 2026Article
- Spectral-acoustic-coordinated astigmatic metalens for wide field-of-view and high spatiotemporal resolution 3D imaging.Light, science & applications · 2026Article
- Dual-view microscopy of single-molecule dipole orientations.Biophysical reports · 2025Article
- Cryogenic light microscopy of vitrified samples with angstrom precision.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Observation of helical pulses.Nature communications · 2025Article
- Single-shot Stokes polarimetry of plasmon-coupled single-molecule fluorescence.Nanophotonics (Berlin, Germany) · 2025Article
- Discriminating single-molecule binding events from diffraction-limited fluorescence.Nature communications · 2025Article
- Observing biological spatio-angular structures and dynamics with statistical image reconstruction and polarized fluorescence microscopy.bioRxiv : the preprint server for biology · 2025Article
- Single molecule orientation and localization microscopy.Nature photonics · 2025Article
- Dipole Determination by Polarimetric Spectroscopy Yielding the Orientation of Gold Nanorods.Small science · 2025Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Estimating the orientation and 3D position of rotationally constrained emitters with localization microscopy typically requires polarization splitting or a large engineered Point Spread Function (PSF). Here we utilize a compact modified PSF for single molecule emitter imaging to estimate simultaneously the 3D position, dipole orientation, and degree of rotational constraint from a single 2D image. We use an affordable and commonly available phase plate, normally used for STED microscopy in the excitation light path, to alter the PSF in the emission light path. This resulting Vortex PSF does not require polarization splitting and has a compact PSF size, making it easy to implement and combine with localization microscopy techniques. In addition to a vectorial PSF fitting routine we calibrate for field-dependent aberrations which enables orientation and position estimation within 30% of the Cramér-Rao bound limit over a 66 μm field of view. We demonstrate this technique on reorienting single molecules adhered to the cover slip, λ-DNA with DNA intercalators using binding-activated localization microscopy, and we reveal periodicity on intertwined structures on supercoiled DNA.
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