Evidence map›Paper›PMID 34635231›Full record

ArticleTalanta2022

SARS-CoV-2 detection with aptamer-functionalized gold nanoparticles.

Srivatsa Aithal, Sarah Mishriki, Rohit Gupta, Rakesh P Sahu, George Botos, Shazia Tanvir, Russell W Hanson, Ishwar K Puri

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Talanta, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
4.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 77 citations in OpenAlex.

  1. Article
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  11. Review
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  14. Review
  15. Article
  16. Aptamers 101: aptamer discovery andChemical science · 2023
    Review
  17. Review
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Srivatsa AithalDepartment of Mechanical Engineering, McMaster University, Hamilton, Ontario, Canada.
Sarah MishrikiSchool of Biomedical Engineering, McMaster University, Hamilton, Ontario, Canada.
Rohit GuptaDepartment of Mechanical Engineering, McMaster University, Hamilton, Ontario, Canada.
Rakesh P SahuDepartment of Mechanical Engineering, McMaster University, Hamilton, Ontario, Canada; School of Biomedical Engineering, McMaster University, Hamilton, Ontario, Canada; Department of Materials Science and Engineering, McMaster University, Hamilton, Ontario, Canada.
George BotosGenemis Laboratories, Cambridge, Ontario, Canada; Aptavid, New York, USA.
Shazia TanvirGenemis Laboratories, Cambridge, Ontario, Canada.
Russell W HansonAptavid, New York, USA.
Ishwar K PuriDepartment of Mechanical Engineering, McMaster University, Hamilton, Ontario, Canada; School of Biomedical Engineering, McMaster University, Hamilton, Ontario, Canada; Department of Materials Science and Engineering, McMaster University, Hamilton, Ontario, Canada. Electronic address: ikpuri@mcmaster.ca.
McMaster University · CABridge University · SS

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A rapid detection test for SARS-CoV-2 is urgently required to monitor virus spread and containment. Here, we describe a test that uses nanoprobes, which are gold nanoparticles functionalized with an aptamer specific to the spike membrane protein of SARS-CoV-2. An enzyme-linked immunosorbent assay confirms aptamer binding with the spike protein on gold surfaces. Protein recognition occurs by adding a coagulant, where nanoprobes with no bound protein agglomerate while those with sufficient bound protein do not. Using plasmon absorbance spectra, the nanoprobes detect 16 nM and higher concentrations of spike protein in phosphate-buffered saline. The time-varying light absorbance is examined at 540 nm to determine the critical coagulant concentration required to agglomerates the nanoprobes, which depends on the protein concentration. This approach detects 3540 genome copies/μl of inactivated SARS-CoV-2.

Indexed as

COVID-19Metal NanoparticlesGoldHumansOligonucleotidesSARS-CoV-2GoldOligonucleotidesAptamerBiosensingGold nanoparticleSARS-CoV-2Spike proteinSurface plasmon resonance

Identifiers

PMID34635231
PMCPMC8409056
OpenAlexW3196876041

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.