ArticleMolecular plant pathology2022
Targeting of SPCSV-RNase3 via CRISPR-Cas13 confers resistance against sweet potato virus disease.
Article in Molecular plant pathology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- First systematic review of the last 30 years of research on sweetpotato: elucidating the frontiers and hotspots.Frontiers in plant science · 2024Pooled it
- Molecular Targets and Trait Innovation in CRISPR-Edited Vegetable Crops: An Up-to-Date Review (2020-2026).Plants (Basel, Switzerland) · 2026Review
- Review
- Genome Editing in Root and Tuber Crop Development in Sub-Saharan Africa.Plant-environment interactions (Hoboken, N.J.) · 2026Review
- Biotic Stress Resistance in Sweet Potato: Mechanisms, Perspectives, and Sustainable Production Strategies.Plants (Basel, Switzerland) · 2026Review
- Establishment of Efficient CRISPR-Cas9 PEG-Mediated DNA-Free Genome Editing Through Ribonucleoproteins Method in Hexaploid Sweetpotato (Plants (Basel, Switzerland) · 2026Article
- Key Sweet Potato Viruses in Fujian Province and Their Distribution, Harmfulness, and Implications in China.Current issues in molecular biology · 2025Article
- Establishing an Immune System Conferring DNA and RNA Virus Resistance in Plants Using CRISPR/Cas12a Multiplex Gene Editing.Plant direct · 2025Article
- Accelerating crop improvement via integration of transcriptome-based network biology and genome editing.Planta · 2025Review
- dCas-Based Tools to Visualize Chromatin or Modify Epigenetic Marks at Specific Plant Genomic Loci.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Systemic evaluation of various CRISPR/Cas13 orthologs for knockdown of targeted transcripts in plants.Genome biology · 2024Article
- CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants.Plants (Basel, Switzerland) · 2024Review
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
- Resistance of the CRISPR-Cas13a Gene-Editing System to Potato Spindle Tuber Viroid Infection in Tomato andViruses · 2024Article
- Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and Animals.International journal of molecular sciences · 2024Review
- Article
- A comprehensive overview of omics-based approaches to enhance biotic and abiotic stress tolerance in sweet potato.Horticulture research · 2024Article
- High-throughput characterization and phenotyping of resistance and tolerance to virus infection in sweetpotato.Virus research · 2024Article
- Review
- Advances and Prospects of Virus-Resistant Breeding in Tomatoes.International journal of molecular sciences · 2023Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sweet potato (Ipomoea batatas) is one of the most important crops in the world, and its production rate is mainly decreased by the sweet potato virus disease (SPVD) caused by the co-infection of sweet potato chlorotic stunt virus (SPCSV) and sweet potato feathery mottle virus. However, methods for improving SPVD resistance have not been established. Thus, this study aimed to enhance SPVD resistance by targeting one of its important pathogenesis-related factors (i.e., SPCSV-RNase3) by using the CRISPR-Cas13 technique. First, the RNA targeting activity of four CRISPR-Cas13 variants were compared using a transient expression system in Nicotiana benthamiana. LwaCas13a and RfxCas13d had more efficient RNA and RNA virus targeting activity than PspCas13b and LshCas13a. Driven by the pCmYLCV promoter for the expression of gRNAs, RfxCas13d exhibited higher RNA targeting activity than that driven by the pAtU6 promoter. Furthermore, the targeting of SPCSV-RNase3 using the LwaCas13a system inhibited its RNA silencing suppressor activity and recovered the RNA silencing activity in N. benthamiana leaf cells. Compared with the wild type, transgenic N. benthamiana plants carrying an RNase3-targeted LwaCas13a system exhibited enhanced resistance against turnip mosaic virus TuMV-GFP and cucumber mosaic virus CMV-RNase3 co-infection. Moreover, transgenic sweet potato plants carrying an RNase3-targeted RfxCas13d system exhibited substantially improved SPVD resistance. This method may contribute to the development of SPVD immune germplasm and the enhancement of sweet potato production in SPVD-prevalent regions.
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