Evidence map›Paper›PMID 34632548›Full record

ArticleApplied biochemistry and biotechnology2021

Construction of Stable T7 Expression System in Saccharomyces cerevisiae by Improving Nuclear Membrane Permeability with Viroporin HIV-1 Vpu.

Kun Yan, Jun Li, Wenya Wang, Qiang Li

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied biochemistry and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Kun YanCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
Jun LiCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.
Wenya WangCollege of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China. wangwy@mail.buct.edu.cn.
Qiang LiDepartment of Chemical Engineering, Key Laboratory for Industrial Biocatalysis, Ministry of Education, Tsinghua University, Beijing, 100084, China.
Beijing University of Chemical Technology · CNTsinghua University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

T7 expression system (T7 RNA polymerase / T7 promoter), derived from T7 bacteriophage, is one of the most extensively used protein expression systems, which is also an enabling tool in synthetic biology. However, in eukaryote, most of T7 expression system is transient expression system. This is mainly due to the absence of post-transcriptional processing of mRNAs transcribed by T7RNAP in eukaryotic cells, so they cannot effectively pass through nuclear membrane and enter cytoplasm. In this study, Saccharomyces cerevisiae was selected as host to construct stable T7 expression system, in which HIV-1 viroporin (Vpu) was used to improve the permeability of nuclear membrane. Results of NanoLuc® (Nluc) luciferase expression indicated that Vpu could effectively promote the transport of T7 transcripts and increase the amount of protein synthesized. The method of using viroporin to improve permeability of the nuclear membrane provides an effective tool for constructing a stable T7 expression system in eukaryote.

Indexed as

Cell MembraneCell Membrane PermeabilityDNA-Directed RNA PolymerasesHuman Immunodeficiency Virus ProteinsSaccharomyces cerevisiaeViral ProteinsViral Regulatory and Accessory ProteinsViroporin ProteinsHIV-1Recombinant Proteinsbacteriophage T7 RNA polymeraseDNA-Directed RNA PolymerasesHuman Immunodeficiency Virus ProteinsRecombinant ProteinsViral ProteinsViral Regulatory and Accessory ProteinsViroporin Proteinsvpu protein, Human immunodeficiency virus 1EukaryoteProtein synthesisSaccharomyces cerevisiaeT7 expression systemViroporin

Identifiers

PMID34632548
PMCPMC8502630
OpenAlexW3206131794

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.