ArticleSTAR protocols2021
A protocol to quantify chromatin compaction with confocal and super-resolution microscopy in cultured cells.
Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Benchmarking three simple DNA staining-based image metrics for live-cell tracking of chromatin organization.Nucleus (Austin, Tex.) · 2026Article
- CDK12/13 inhibition overcomes platinum resistance by impairing RNA metabolism.Signal transduction and targeted therapy · 2026Article
- Tracking DNA damage localization and chromatin remodeling in live cells using time-resolved quantitative analysis of DNA counterstains.Cellular and molecular life sciences : CMLS · 2026Article
- Lamin A/C protects chromatin accessibility during mechanical loading in human skeletal muscle.Cell communication and signaling : CCS · 2025Article
- XPO1 R749Q Mutations Co-occur with POLE Mutations in Cancer and Can Be Targeted to Overcome Chemoresistance.Cancer research · 2025Article
- Differentiation Stage Predicts Radiosensitivity in Mesenchymal-Like Pancreatic Cancer.International journal of radiation oncology, biology, physics · 2025Article
- Herpes simplex virus type 1 reshapes host chromatin architecture via transcription machinery hijacking.Nature communications · 2025Article
- Digitalized organoids: integrated pipeline for high-speed 3D analysis of organoid structures using multilevel segmentation and cellular topology.Nature methods · 2025Article
- A highly conserved neuronal microexon in DAAM1 controls actin dynamics, RHOA/ROCK signaling, and memory formation.Nature communications · 2025Article
- Regulation of chromatin modifications through coordination of nucleus size and epithelial cell morphology heterogeneity.Communications biology · 2025Article
- Colloidal few layered graphene-tannic acid preserves the biocompatibility of periodontal ligament cells.Beilstein journal of nanotechnology · 2025Article
- Functional specificity of liquid-liquid phase separation at the synapse.Nature communications · 2024Article
- Heterochromatin protein 1 alpha (HP1α) undergoes a monomer to dimer transition that opens and compacts live cell genome architecture.Nucleic acids research · 2024Article
- Probing chromatin condensation dynamics in live cells using interferometric scattering correlation spectroscopy.Communications biology · 2024Article
- Microscopy methods to visualize nuclear organization in biomechanical studies.Current opinion in biomedical engineering · 2024Article
- LATS1 controls CTCF chromatin occupancy and hormonal response of 3D-grown breast cancer cells.The EMBO journal · 2024Article
- Article
- Depletion of lamins B1 and B2 promotes chromatin mobility and induces differential gene expression by a mesoscale-motion-dependent mechanism.Genome biology · 2024Article
- Coupling of Perinuclear Actin Cap and Nuclear Mechanics in Regulating Flow-Induced Yap Spatiotemporal Nucleocytoplasmic Transport.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- A deep learning method that identifies cellular heterogeneity using nanoscale nuclear features.Nature machine intelligence · 2024Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we describe three complementary microscopy-based approaches to quantify morphological changes of chromatin organization in cultured adherent cells: the analysis of the coefficient of variation of DNA, the measurement of DNA-free nuclear areas, and the quantification of chromatin-associated proteins at the nuclear edge. These approaches rely on confocal imaging and stochastic optical reconstruction microscopy and allow a fast and robust quantification of chromatin compaction. These approaches circumvent inter-variability between imaging conditions and apply to every type of adherent cells. For complete details on the use and execution of this protocol, please refer to Neguembor et al. (2021).
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