Evidence map›Paper›PMID 34632411›Full record

ArticleSTAR protocols2021

Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2.

Hector Huerga Encabo, Rachel Ulferts, Aneesh Sharma, Rupert Beale, Dominique Bonnet

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. CRISPR/dCas9 DNA methylation editing is heritable during human hematopoiesis and shapes immune progeny.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Hector Huerga EncaboHematopoietic Stem Cell Laboratory, The Francis Crick Institute, London, NW1 1AT, UK.
Rachel UlfertsCell Biology of Infection Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
Aneesh SharmaHematopoietic Stem Cell Laboratory, The Francis Crick Institute, London, NW1 1AT, UK.
Rupert BealeCell Biology of Infection Laboratory, The Francis Crick Institute, London NW1 1AT, UK.
Dominique BonnetHematopoietic Stem Cell Laboratory, The Francis Crick Institute, London, NW1 1AT, UK.
The Francis Crick Institute · GB

Funding

Cancer Research UK FC001045Wellcome TrustWellcome Trust FC001045
6 · The paper itself

Abstract

Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs, inlcuding procedures for SARS-CoV-2 production and titration, isolation of human HSPCs from different sources (bone marrow, umbilical cord, or peripheral blood), and quantification of SARS-Cov-2 infection capacity by RT-qPCR and colony forming unit assay. For complete details on the use and execution of this protocol, please refer to Huerga Encabo et al. (2021).

Indexed as

Bone MarrowColony-Forming Units AssayCOVID-19COVID-19 Nucleic Acid TestingFetal BloodHematopoietic Stem CellsHumansSARS-CoV-2Cell-based AssaysCell BiologyCell isolationImmunologyMicrobiologyMicroscopyStem Cells

Identifiers

PMID34632411
PMCPMC8492889
OpenAlexW3207336721

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.