Evidence map›Paper›PMID 34632220›Full record

ArticleACS omega2021

Amplification of Femtograms of Bacterial DNA Within 3 h Using a Digital Microfluidics Platform for MinION Sequencing.

Yuguang Liu, Patricio Jeraldo, Helena Mendes-Soares, Thao Masters, Abigail E Asangba, Heidi Nelson, Robin Patel, Nicholas Chia, Marina Walther-Antonio

Open access · goldAbstract read
In one paragraph

Article in ACS omega, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 25 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. DNA storage: The future direction for medical cold data storage.Synthetic and systems biotechnology · 2025
    Review
  5. DNA-DISK: Automated end-to-end data storage via enzymatic single-nucleotide DNA synthesis and sequencing on digital microfluidics.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  6. Article
  7. Review
  8. Review
  9. "Omics" Techniques Used in Marine Biofouling Studies.International journal of molecular sciences · 2023
    Review
  10. Review
  11. Article
  12. Review
  13. Microbial Single-Cell Analysis: What Can We Learn From Mammalian?Frontiers in cell and developmental biology · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Yuguang LiuDepartment of Surgery, Division of Surgical Research, Mayo Clinic, Rochester, Minnesota 55905-0002, United States.
Patricio JeraldoDepartment of Surgery, Division of Surgical Research, Mayo Clinic, Rochester, Minnesota 55905-0002, United States.ORCID https://orcid.org/0000-0003-0282-4217
Helena Mendes-SoaresMicrobiome Program, Center for Individualized Medicine, Mayo Clinic, Rochester, Minnesota 55905-0002, United States.
Thao MastersDivision of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905-0002, United States.
Abigail E AsangbaDepartment of Surgery, Division of Surgical Research, Mayo Clinic, Rochester, Minnesota 55905-0002, United States.
Heidi NelsonDepartment of Surgery, Division of Surgical Research, Mayo Clinic, Rochester, Minnesota 55905-0002, United States.
Robin PatelDivision of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905-0002, United States.
Nicholas ChiaDepartment of Surgery, Division of Surgical Research, Mayo Clinic, Rochester, Minnesota 55905-0002, United States.
Marina Walther-AntonioDepartment of Surgery, Division of Surgical Research, Mayo Clinic, Rochester, Minnesota 55905-0002, United States.ORCID https://orcid.org/0000-0002-5531-3712
Mayo Clinic · US

Funding

Use of microfluidic tumor cultures to enable clinical trials of therapies for ovarian cancerP50CA136393 · NCI · MAYO CLINIC ROCHESTER · PI ELLEN L. GOODE · 2009 to 2026
$37.0M
ZESTRIL VS. VERAPAMIL IN THE TREATMENT OF HYPERTENSIONM01RR000585 · NCRR · MAYO CLINIC ROCHESTER · PI RIZZA, ROBERT A. · 1985 to 2006
$30.4M
Institutional Career Development CoreKL2TR002379 · NCATS · MAYO CLINIC ROCHESTER · PI NILUFER ERTEKIN-TANER · 2017 to 2026
$14.9M
Musculoskeletal Research Training ProgramT32AR056950 · NIAMS · MAYO CLINIC ROCHESTER · PI Jennifer J Westendorf · 2009 to 2026
$7.0M
NCATS NIH HHS KL2 TR002379NCI NIH HHS P50 CA136393NCRR NIH HHS M01 RR000585NIAMS NIH HHS T32 AR056950
6 · The paper itself

Abstract

Whole genome sequencing is emerging as a promising tool for the untargeted detection of a broad range of microbial species for diagnosis and analysis. However, it is logistically challenging to perform the multistep process from sample preparation to DNA amplification to sequencing and analysis within a short turnaround time. To address this challenge, we developed a digital microfluidic device for rapid whole genome amplification of low-abundance bacterial DNA and compared results with conventional in-tube DNA amplification. In this work, we chose

Identifiers

PMID34632220
PMCPMC8495859
OpenAlexW3204658235

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.