ArticleFrontiers in immunology2021
Chicken DDX1 Acts as an RNA Sensor to Mediate IFN-β Signaling Pathway Activation in Antiviral Innate Immunity.
Article in Frontiers in immunology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Pigeon employs a conserved IRF7 gene to mediate innate immune response.Poultry science · 2026Article
- Genetic basis of immunity in Indian cattle as revealed by comparative analysis of Bos genome.Scientific reports · 2026Article
- DTMUV upregulates DDX17 expression to facilitate viral replication.Veterinary research · 2025Article
- Exploring the genetic basis of Newcastle disease virus in chickens: a comprehensive review.Frontiers in immunology · 2025Review
- Pattern recognition receptors and their roles in antiviral innate immunity in livestock.Frontiers in immunology · 2025Review
- Identification of pigeon mitochondrial antiviral signaling protein (MAVS) and its role in antiviral innate immunity.Archives of virology · 2024Article
- Genome-wide investigation to assess copy number variants in the Italian local chicken population.Journal of animal science and biotechnology · 2024Article
- Interaction of Teleost Fish TRPV4 with DEAD Box RNA Helicase 1 Regulates Iridovirus Replication.Journal of virology · 2023Article
- Pathologic Mechanisms of the Newcastle Disease Virus.Viruses · 2023Review
- Chicken IFI6 inhibits avian reovirus replication and affects related innate immune signaling pathways.Frontiers in microbiology · 2023Article
- Chicken miR-126-5p negatively regulates antiviral innate immunity by targeting TRAF3.Veterinary research · 2022Article
- DDX5/METTL3-METTL14/YTHDF2 Axis Regulates Replication of Influenza A Virus.Microbiology spectrum · 2022Article
- Emerging role of RNA sensors in tumor microenvironment and immunotherapy.Journal of hematology & oncology · 2022Review
- Functional characterization of goose IRF1 in IFN induction and anti-NDV infection.Veterinary research · 2022Article
- Screening and identification of nucleocapsid protein-nanobodies that inhibited Newcastle disease virus replication in DF-1 cells.Frontiers in microbiology · 2022Article
- Bat Employs a Conserved MDA5 Gene to Trigger Antiviral Innate Immune Responses.Frontiers in immunology · 2022Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chickens are the natural host of Newcastle disease virus (NDV) and avian influenza virus (AIV). The discovery that the RIG-I gene, the primary RNA virus pattern recognition receptor (PRR) in mammals, is naturally absent in chickens has directed attention to studies of chicken RNA PRRs and their functions in antiviral immune responses. Here, we identified Asp-Glu-Ala-Asp (DEAD)-box helicase 1 (DDX1) as an essential RNA virus PRR in chickens and investigated its functions in anti-RNA viral infections. The chDDX1 gene was cloned, and cross-species sequence alignment and phylogenetic tree analyses revealed high conservation of DDX1 among vertebrates. A quantitative RT-PCR showed that chDDX1 mRNA are widely expressed in different tissues in healthy chickens. In addition, chDDX1 was significantly upregulated after infection with AIV, NDV, or GFP-expressing vesicular stomatitis virus (VSV-GFP). Overexpression of chDDX1 in DF-1 cells induced the expression of IFN-β, IFN-stimulated genes (ISGs), and proinflammatory cytokines; it also inhibited NDV and VSV replications. The knockdown of chDDX1 increased the viral yield of NDV and VSV and decreased the production of IFN-β, which was induced by RNA analog polyinosinic-polycytidylic acid (poly[I:C]), by AIV, and by NDV. We used a chicken IRF7 (chIRF7) knockout DF-1 cell line in a series of experiments to demonstrate that chDDX1 activates IFN signaling
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