Evidence map›Paper›PMID 34629514›Full record

ArticleJournal of food science and technology2021

Rapid porcine detection in gelatin-based highly processed products using loop mediated isothermal amplification.

Nor Asmara Tasrip, Mohd Nasir Mohd Desa, Nur Fadhilah Khairil Mokhtar, Nurhayatie Sajali, Amalia Mohd Hashim, Md Eaqub Ali, Cheah Yoke Kqueen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of food science and technology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. A species-specific triplex PCR assay for authentication ofMitochondrial DNA. Part B, Resources · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 3 countries.

Nor Asmara TasripHalal Products Research Institute, Universiti Putra Malaysia, 43400 Serdang, Selangor Malaysia.
Mohd Nasir Mohd DesaHalal Products Research Institute, Universiti Putra Malaysia, 43400 Serdang, Selangor Malaysia.ORCID 0000-0003-0200-4796
Nur Fadhilah Khairil MokhtarHalal Products Research Institute, Universiti Putra Malaysia, 43400 Serdang, Selangor Malaysia.
Nurhayatie SajaliHalal Products Research Institute, Universiti Putra Malaysia, 43400 Serdang, Selangor Malaysia.
Amalia Mohd HashimMinistry of Higher Education, Konsortium Institut Halal IPT Malaysia, 62200 Putrajaya, Malaysia.
Md Eaqub AliModesto City Schools, 426 Locust St., Modesto, CA 95351-2699 USA.
Cheah Yoke KqueenDepartment of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Malaysia.
Universiti Putra Malaysia · MYUniversity of Malaya · MY

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Low DNA concentration recovered from highly processed products such as gelatin and gelatin-based products renders difficulty in detecting porcine contamination using conventional PCR techniques. We documented here a porcine-specific loop-mediated isothermal amplification (LAMP) to identify porcine traces in gelatin products. The porcine-specific primers were designed according to mitochondrial DNA of Cytochrome b gene sequence. Here we used two different reaction mixtures for LAMP assay (GENIE and MYRM) against the same DNA samples extracted from gelatin products and porcine-specific primers to detect the presence of porcine DNA. The porcine-specific primers were shown to be specific only to SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at (10.1007/s13197-020-04932-2).

Indexed as

CalceinFluorescent dyeGelatin authenticationLAMPLoop isothermal amplificationPorcine DNAReal-time LAMP

Identifiers

PMID34629514
PMCPMC8478990
OpenAlexW3120199661

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.