Evidence map›Paper›PMID 34628631›Full record

ArticleAdvances in experimental medicine and biology2021

Practical Considerations in Method Development for Gas Chromatography-Based Metabolomic Profiling.

Andre Cunha Paiva, Amilton Moreira de Oliveira, Juliana Crucello, Roselaine Facanali, Leandro Wang Hantao

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Article in Advances in experimental medicine and biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Andre Cunha PaivaInstitute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Amilton Moreira de OliveiraInstitute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Juliana CrucelloInstitute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Roselaine FacanaliInstitute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Leandro Wang HantaoInstitute of Chemistry, University of Campinas (UNICAMP), Campinas, SP, Brazil. wang@unicamp.br.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter discusses the fundamentals of gas chromatography (GC) to improve method development for metabolic profiling of complex biological samples. The selection of column geometry and phase ratio impacts analyte mass transfer, which must be carefully optimized for fast analysis. Stationary phase selection is critical to obtain baseline resolution of critical pairs, but such selection must consider important aspects of metabolomic protocols, such as derivatization and dependence of analyte identification on existing databases. Sample preparation methods are also addressed depending on the sample matrix, including liquid-liquid extraction and solid-phase microextraction.

Indexed as

MetabolomicsChromatography, GasComprehensive two-dimensional gas chromatographyLiquid-liquid extractionMass spectrometryMetabolic profilingMetabolomicsSample preparationUntargeted analysis

Identifiers

PMID34628631

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.